Grant: Research Tools and Instruments - Category 1 (<$150,000)

A microsensor system consisting of microelectrodes for the measurement of O2, pH and redox, a picoammeter (for O2, H2, H2S, NO3, NO2 ), a millivoltmeter (pH and redox), and micromanipulation accessories will be used for microenvironmental analysis (spatial resolution of about 20µm) of microbial structures.   The microsensor system expands our range of techniques and methods that have been developed at Ryerson for the study of floc and film structure, composition and properties of extracellular polymers, and molecular ecology. Our strategic focus on microbial structures has provided information relevant to industry, and has led for example to significant input into the design, operation of biological wastewater treatment including submerged membrane bioreactors for solids separation,  membrane aerated bioreactors, and hybrid fixed-film activated sludge systems with our industrial partners. The microsensor system will assist with the generation of both fundamental and applied information on biological processes in engineered systems, including membrane-based bioreactors, and to improve upon existing tools.  This is increasingly important to advances in environmental control, biorefining and understanding key environmental processes.  We employ advanced analytical and experimental approaches that positions Civil and Chemical Engineering to develop new technologies and adapt advances in microbiology and biotechnology. The microsensor system will be an important tool for the Environmental Interface and Biofilms Laboratory. Most importantly our students, highly qualified personnel, and partners will be the main beneficiaries as they will have access to state-of-the art facilities and will acquire important knowledge, skills and abilities.