Grant: Discovery Grants Program - Individual
Queen's University
$70,000.00
Microstructured fibres (MSFs) for nanoelectrospray ionization (nano-esi) and chromatographic applications
FY 2012-13
Summary
Our laboratory is interested in the development of novel separation and detection methods for chemical analysis. Chemical analysis forms the basis for discovery in a wide variety of disciplines (e.g. environment, medical and ""omics"" related research areas). The development of analysis systems that are faster, require less sample with increased sensitivity are in constant demand and become critical to probing e.g. biological and chemical systems at cellular/sub-cellular levels. We plan to adopt a novel optical fiber material for use as both a substrate for open tubular liquid chromatography and for high sensitivity nanoelectrospray ionization mass spectrometry. Fibres with a number of different hole sizes and hole patterns have only recently become commercially available. The multiple, parallel silica channels presented by the MSF act as a promising substrate for a nanoOTLC column, as they have diameters near the ideal range for interactions (1 to 3 µm). Furthermore, the application of MSF's for nanoelectrospray has the potential to substantially improve the way in which nanoelectrospray is performed. Conventional emitters are tapered and as a result suffer from continuous clogging and offer very poor emitter-emitter reproducibility. MSF's can function as an emitter by pumping liquid through the pores of the fibre and as a result of the numerous flow paths virtually eliminate problems associated with clogging. Furthermore, if each hole could act as an independent emitter then a significant increase in ion production and sensitivity would result. As such, a greater number of electrospray emitters increase the ionization efficiency leading to enhanced sensitivity. MSFs with different hole patterns and hole sizes will be utilized to manufacture nanoelectrospray emitters that both improve nanospray robustness and enhance nanoelectrospray sensitivity. The application of these novel fibres to both liquid chromatography and nanoelectrospray has the potential to completely revolutionize both research areas.
Program
Discovery Grants Program - Individual
Recipient
Location
Ontario, Canada
Recipient Type
Academia
Type
Grant
Source
NSERC's Awards Data
As published
Application id
239026-2011
Name nom
Oleschuk, Richard
Department département
Chemistry
Organization id
29
Institution établissement
Queen's University
Province en
Ontario
Province fr
Ontario
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2012
Competition year année de concours
2011
Award amount
70000
Program id
RGPIN
Program name en
Discovery Grants Program - Individual
Program name fr
Programme de subventions à la découverte - individuelles
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
1504
Committee name en
Chemistry
Committee name fr
Chimie
Area of application code
515
Area of application group en
Manufacturing processes and products
Area of application group fr
Procédés et produits industriels
Area of application en
Instrumentation technology
Area of application fr
Technologie de l'instrumentation
Research subject code
3500
Research subject group en
Analytical chemistry
Research subject group fr
Chimie analytique
Research subject en
Analytical chemistry
Research subject fr
Chimie analytique
Partie
2
Nb partie
5
Application title
Microstructured fibres (MSFs) for nanoelectrospray ionization (nano-esi) and chromatographic applications
Keyword
NSERC
Application summary
Our laboratory is interested in the development of novel separation and detection methods for chemical analysis. Chemical analysis forms the basis for discovery in a wide variety of disciplines (e.g. environment, medical and ""omics"" related research areas). The development of analysis systems that are faster, require less sample with increased sensitivity are in constant demand and become critical to probing e.g. biological and chemical systems at cellular/sub-cellular levels. We plan to adopt a novel optical fiber material for use as both a substrate for open tubular liquid chromatography and for high sensitivity nanoelectrospray ionization mass spectrometry. Fibres with a number of different hole sizes and hole patterns have only recently become commercially available. The multiple, parallel silica channels presented by the MSF act as a promising substrate for a nanoOTLC column, as they have diameters near the ideal range for interactions (1 to 3 µm). Furthermore, the application of MSF's for nanoelectrospray has the potential to substantially improve the way in which nanoelectrospray is performed. Conventional emitters are tapered and as a result suffer from continuous clogging and offer very poor emitter-emitter reproducibility. MSF's can function as an emitter by pumping liquid through the pores of the fibre and as a result of the numerous flow paths virtually eliminate problems associated with clogging. Furthermore, if each hole could act as an independent emitter then a significant increase in ion production and sensitivity would result. As such, a greater number of electrospray emitters increase the ionization efficiency leading to enhanced sensitivity. MSFs with different hole patterns and hole sizes will be utilized to manufacture nanoelectrospray emitters that both improve nanospray robustness and enhance nanoelectrospray sensitivity. The application of these novel fibres to both liquid chromatography and nanoelectrospray has the potential to completely revolutionize both research areas.
