Grant: Engage Grants Program
Brock University
$25,000.00
Development of potential alternative fluorophores to Sybr Green to stain double stranded DNA******
FY 2018-19
Summary
Fluorescent dyes that emit light upon binding to double stranded DNA are crucial in applications such as quantitative Polymerase Chain Reactions (qPCR) and High Resolution Melt (HRM) experiments, both important techniques in molecular biology. These techniques have found a wide range of applications in life science, forensics, human health and beyond. While the dyes that are currently in use fulfill the requirements for qPCR and HRM to various degrees, many are expensive or not efficient enough for the applications. This project will investigate alternative dyes based on 1,8-naphthalimide core structures. The 1,8-naphthalimide fluorescent dyes are usually straightforward to synthesize, and some analogs have unique fluorescent properties that are suitable for qPCR and HRM applications. This research will generate 1,8-naphthalimide dyes that are readily available, and potentially suitable for both qPCR and HRM applications.
Program
Engage Grants Program
Recipient
Location
Ontario, Canada
Recipient Type
Academia
Type
Grant
Source
NSERC's Awards Data
As published
Application id
534231-2018
Name nom
Yan, Hongbin
Department département
Chemistry
Organization id
22
Institution établissement
Brock University
Province en
Ontario
Province fr
Ontario
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2018
Competition year année de concours
2018
Award amount
25000
Program id
EGP
Program name en
Engage Grants Program
Program name fr
Programme de subventions d'engagement partenariat
Group en
Research partnerships programs
Group fr
Programmes de partenariat
Committee code
1553
Committee name en
Ontario Internal Decision Committee
Committee name fr
Comité de décision interne pour l'Ontario
Area of application code
1201
Area of application group en
Advancement of knowledge
Area of application group fr
Avancement des connaissances
Area of application en
Life sciences (including biotechnology)
Area of application fr
Sciences de la vie (y compris biotechnologie)
Research subject code
3705
Research subject group en
Organic chemistry
Research subject group fr
Chimie organique
Research subject en
Bio-organic chemistry
Research subject fr
Chimie bio-organique
Partie
1
Nb partie
1
Application title
Development of potential alternative fluorophores to Sybr Green to stain double stranded DNA******
Keyword
NSERC
Application summary
Fluorescent dyes that emit light upon binding to double stranded DNA are crucial in applications such as quantitative Polymerase Chain Reactions (qPCR) and High Resolution Melt (HRM) experiments, both important techniques in molecular biology. These techniques have found a wide range of applications in life science, forensics, human health and beyond. While the dyes that are currently in use fulfill the requirements for qPCR and HRM to various degrees, many are expensive or not efficient enough for the applications. This project will investigate alternative dyes based on 1,8-naphthalimide core structures. The 1,8-naphthalimide fluorescent dyes are usually straightforward to synthesize, and some analogs have unique fluorescent properties that are suitable for qPCR and HRM applications. This research will generate 1,8-naphthalimide dyes that are readily available, and potentially suitable for both qPCR and HRM applications.
