Grant: Project Grant
University of British Columbia
$100,000.00
Functional characterization of genomic DNA elements regulating epigenetic silencing
FY 2016-17
Summary
The sex chromosomes are different between males and females. While males have one X chromosome and the sex-determining Y chromosome females have two X chromosomes. Since the X chromosome has almost 1000 genes there would be a considerable dosage imbalance if one X chromosome was not silenced in females. This silencing of one X in females is called X-chromosome inactivation and is random early in development so that females are mosaic for having one or the other X chromosome active. Almost a quarter of the genes on the X chromosome are not completely silenced and we will delete regions across these genes in mouse embryonic stem cells to identify the regions of the DNA that permit these gene to be expressed on an otherwise inactive X chromosome
Department
Program
Project Grant
Recipient
Location
British Columbia, Canada
Recipient Type
Academia
Type
Grant
Source
CIHR Funding Decisions Database
As published
Id
366630
Name
Brown Carolyn J
Region
British Columbia
Country
Canada
Orgtype
Institution Paid; Research Institution
Instname2
Genetics
Theme2
Biomedical
Prcname2
Project Grant Competition
Projecttitle
Functional characterization of genomic DNA elements regulating epigenetic silencing
Programtype2
Operating Grants
Orgnameinp2
University of British Columbia
Cihramount2
$100,000
Cihrequipment2
$0
Programname2
Project Grant
Keyworddelim
Bac Transgenesis; Dna Methylation; Gene Regulation; Heterochromatin; Mouse Modelling; Sexual Dimorphism; Transcription Factor; X Chromosome; X Inactivation; Yy1
Abstract
The sex chromosomes are different between males and females. While males have one X chromosome and the sex-determining Y chromosome females have two X chromosomes. Since the X chromosome has almost 1000 genes there would be a considerable dosage imbalance if one X chromosome was not silenced in females. This silencing of one X in females is called X-chromosome inactivation and is random early in development so that females are mosaic for having one or the other X chromosome active. Almost a quarter of the genes on the X chromosome are not completely silenced and we will delete regions across these genes in mouse embryonic stem cells to identify the regions of the DNA that permit these gene to be expressed on an otherwise inactive X chromosome
Competitiondate
201611
Approvedterm2
1 yr 0 mth
Pinamesdelim
Brown, Carolyn J
Approvedterm
1 an 0 mois
Cihrcontribution2
~ $100,000~ $0~
