Grant: Research Tools and Instruments - Category 1 (<$150,000)
University of Waterloo
$72,673.00
Two-pump HPLC system
FY 2006-07
Summary
The equipment requested is a high-performance liquid chromatography system (HPLC) equipped with two high-pressure pumps to be used in the research on comprehensive two-dimensional liquid chromatography (LC?LC) and very high efficiency HPLC. LC?LC is a new technique for the separation of very complex mixtures (containing hundreds or thousands of individual components), including many natural samples. In this technique, a sample is introduced to an HPLC column, where its components undergo preliminary separation. As material elutes from this column, small aliquots of it are repeatedly injected into a second column packed with a different stationary phase, where additional, fast separation takes place. As a result, the separation power of LC?LC is much greater than that of conventional, one-dimensional HPLC. The new equipment will be used to initiate the research in the area of HPLC in my group. So far we have specialized in gas chromatography, a technique where a gas rather than a solvent carries the sample components along the column. This approach, however, is limited to compounds with reasonably high vapour pressures. HPLC is a complementary technique, allowing the analysis of non-volatile or thermally unstable compounds. Our background in gas chromatography, and comprehensive two-dimensional gas chromatography in particular, should give us a unique perspective on HPLC, which should help us quickly reach the forefront of HPLC research. The equipment will significantly boost the quality of training of both graduate and undergraduate students in my group, significantly enhancing their competitiveness in the job market.
Program
Research Tools and Instruments - Category 1 (<$150,000)
Recipient
Location
Ontario, Canada
Recipient Type
Academia
Type
Grant
Source
NSERC's Awards Data
As published
Application id
344674-2007
Name nom
Górecki, Tadeusz
Department département
Chemistry
Organization id
33
Institution établissement
University of Waterloo
Province en
Ontario
Province fr
Ontario
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2006
Competition year année de concours
2007
Award amount
72673
Program id
EQPEQ
Program name en
Research Tools and Instruments - Category 1 (<$150,000)
Program name fr
Outils et instruments de recherche - catégorie 1 (<150 000$)
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
26
Committee name en
Anal. & Phys. Chemistry
Committee name fr
Chimie analytique et physique
Area of application code
515
Area of application group en
Manufacturing processes and products
Area of application group fr
Procédés et produits industriels
Area of application en
Instrumentation technology
Area of application fr
Technologie de l'instrumentation
Research subject code
3503
Research subject group en
Analytical chemistry
Research subject group fr
Chimie analytique
Research subject en
Separation methods
Research subject fr
Méthodes de séparation
Partie
1
Nb partie
1
Application title
Two-pump HPLC system
Keyword
NA
Application summary
The equipment requested is a high-performance liquid chromatography system (HPLC) equipped with two high-pressure pumps to be used in the research on comprehensive two-dimensional liquid chromatography (LC?LC) and very high efficiency HPLC. LC?LC is a new technique for the separation of very complex mixtures (containing hundreds or thousands of individual components), including many natural samples. In this technique, a sample is introduced to an HPLC column, where its components undergo preliminary separation. As material elutes from this column, small aliquots of it are repeatedly injected into a second column packed with a different stationary phase, where additional, fast separation takes place. As a result, the separation power of LC?LC is much greater than that of conventional, one-dimensional HPLC. The new equipment will be used to initiate the research in the area of HPLC in my group. So far we have specialized in gas chromatography, a technique where a gas rather than a solvent carries the sample components along the column. This approach, however, is limited to compounds with reasonably high vapour pressures. HPLC is a complementary technique, allowing the analysis of non-volatile or thermally unstable compounds. Our background in gas chromatography, and comprehensive two-dimensional gas chromatography in particular, should give us a unique perspective on HPLC, which should help us quickly reach the forefront of HPLC research. The equipment will significantly boost the quality of training of both graduate and undergraduate students in my group, significantly enhancing their competitiveness in the job market.
