Grant: Discovery Grants Program - Individual
Memorial University of Newfoundland
$25,000.00
Posttranslational modification-specific proteomics approach to identification of molecular biomarkers for wheat seed dormancy control and regulation
FY 2009-10
Summary
Pre-harvest sprouting (PHS) is a perpetual threat to wheat production. Sprouting in wheat can result in massive harvest losses of up to 40% in the price/tonne for producers because sprouted grain is an important downgrading factor. Seed dormancy, which is controlled by genetic and environmental factors, should be strong enough to inhibit germination. PHS resistance is a form of grade protection that is best built into wheat cultivars through genetic resistance. However, still very little is known about the processes and molecular signalling mechanisms occurring during dormancy control. The research program aims to examine sources of wheat seed dormancy and sprouting resistance. I propose the proteomic analysis of dormancy in all aspects - biomarker pattern linking genome to proteome, genetics factors control to specific phenotypic expression plasticity, post-translational signalling, phytohormone-mediated response and effect of environmental changes. In the next five years, in-depth systematic analysis of differential protein correlation profiling including localization, dynamics, and interaction partners will be focused on specific and functionally relevant 'sub-proteomes'. The hybrid lines of doubled haploid populations segregating for dormancy/sprouting resistance will be used to validate the identified candidate biomarker proteins. The research will create new knowledge regarding the biochemical nature of sprouting resistance and dormancy control, will lead to understanding of the biochemical nature for reduced wheat seed dormancy under unfavourable environmental conditions and identify molecular basis and genetic resources for sustained, internationally competitive, variety development. The outcome of this research is the thorough evaluation of germplasm enhanced for sprouting resistance by the systematic proteomic analysis of dormancy biomarkers. The broad basis of the germplasm, derived from elite Canadian genetics, ensures genetic diversity for long term selection as well as for immediate variety development.
Program
Discovery Grants Program - Individual
Recipient
Location
Newfoundland and Labrador, Canada
Recipient Type
Academia
Type
Grant
Source
NSERC's Awards Data
As published
Application id
371787-2009
Name nom
Bykova, Natalia
Department département
Biology
Organization id
89
Institution établissement
Memorial University of Newfoundland
Province en
Newfoundland and Labrador
Province fr
Terre-Neuve-et-Labrador
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2009
Competition year année de concours
2009
Award amount
25000
Program id
RGPIN
Program nane en
Discovery Grants Program - Individual
Program name fr
Programme de subventions à la découverte - individuelles
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
3
Committee name en
Plant Biology & Food Science
Committee name fr
Biol vég/sc alimentaire
Area of application code
1201
Area of application group en
Advancement of knowledge
Area of application group fr
Avancement des connaissances
Area of application en
Life sciences (including biotechnology)
Area of application fr
Sciences de la vie (y compris biotechnologie)
Research subject code
4903
Research subject group en
Plant and treebiology
Research subject group fr
Biologie végétale et des arbres
Research subject en
Plant growth and development
Research subject fr
Croissance et développement des végétaux
Partie
1
Nb partie
1
Application title
Posttranslational modification-specific proteomics approach to identification of molecular biomarkers for wheat seed dormancy control and regulation
Keyword
NA
Application summary
Pre-harvest sprouting (PHS) is a perpetual threat to wheat production. Sprouting in wheat can result in massive harvest losses of up to 40% in the price/tonne for producers because sprouted grain is an important downgrading factor. Seed dormancy, which is controlled by genetic and environmental factors, should be strong enough to inhibit germination. PHS resistance is a form of grade protection that is best built into wheat cultivars through genetic resistance. However, still very little is known about the processes and molecular signalling mechanisms occurring during dormancy control. The research program aims to examine sources of wheat seed dormancy and sprouting resistance. I propose the proteomic analysis of dormancy in all aspects - biomarker pattern linking genome to proteome, genetics factors control to specific phenotypic expression plasticity, post-translational signalling, phytohormone-mediated response and effect of environmental changes. In the next five years, in-depth systematic analysis of differential protein correlation profiling including localization, dynamics, and interaction partners will be focused on specific and functionally relevant 'sub-proteomes'. The hybrid lines of doubled haploid populations segregating for dormancy/sprouting resistance will be used to validate the identified candidate biomarker proteins. The research will create new knowledge regarding the biochemical nature of sprouting resistance and dormancy control, will lead to understanding of the biochemical nature for reduced wheat seed dormancy under unfavourable environmental conditions and identify molecular basis and genetic resources for sustained, internationally competitive, variety development. The outcome of this research is the thorough evaluation of germplasm enhanced for sprouting resistance by the systematic proteomic analysis of dormancy biomarkers. The broad basis of the germplasm, derived from elite Canadian genetics, ensures genetic diversity for long term selection as well as for immediate variety development.
