Grant: Research Tools and Instruments - Category 1 (<$150,000)
University of Ontario Institute of Technology
$40,510.00
Real time PCR for gene expression studies
FY 2009-10
Summary
The requested real time RT-PCR will allow us to perform gene expression studies in bacteria, nematodes, plants, and mammalian cell lines. This equipment is essential for the research of six different research programs, and will also serve as a valuable tool for other research groups at the university.
The Kumar group is investigating mechanisms that regulate the expression of Resistance-Nodulation-cell Division (RND) family efflux pumps in Acinetobacter baumannii. Analyzing expression of RND operons in different A. baumannii isolates will provide valuable information on how these organisms survive in toxic environments aid in designing new drug therapy against this organism. The Forrester laboratory will use the real time RT-PCR to determine the level of expression of Ligand-Gated Chloride Channels (LGCC) genes in different developmental stages of the parasitic nematodes with a long-term objective of developing anti-helminthics. The Bonetta laboratory will utilize the requested equipment to identify mechanisms important for cell wall synthesis by using a genetic approach in model plant Arabidopsis thaliana. A real time RT-PCR will allow detection of genes that are expressed at low levels. The Strap group will use real time RT-PCR equipment to monitor and compare gene expression of extracellular metabolites produced by bacteria in natural and laboratory conditions. Use of the real time RT-PCR instrument by the Desaulniers group will allow for monitoring of the effect of siRNA molecules on the expression of a variety of genes. The Green-Johnson group will use the real time RT-PCR to examine the ability of functional food components to influence the expression of genes encoding cytokines, key cell surface molecules, and eicosanoids.
The requested equipment will be used by six different research programs that are training a large numbers of Highly Qualified Personnels (HQPs) and therefore will become an extremely useful aspect of HQP training at our institution.
Program
Research Tools and Instruments - Category 1 (<$150,000)
Location
Ontario, Canada
Recipient Type
Academia
Type
Grant
Source
NSERC's Awards Data
As published
Application id
390572-2010
Name nom
Kumar, Ayush
Department département
Health Sciences, Faculty of
Organization id
19222
Institution établissement
University of Ontario Institute of Technology
Province en
Ontario
Province fr
Ontario
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2009
Competition year année de concours
2010
Award amount
40510
Program id
EQPEQ
Program nane en
Research Tools and Instruments - Category 1 (<$150,000)
Program name fr
Outils et instruments de recherche - catégorie 1 (<150 000$)
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
1501
Committee name en
Genes, Cells and Molecules
Committee name fr
Gènes, cellules et molécules
Area of application code
1201
Area of application group en
Advancement of knowledge
Area of application group fr
Avancement des connaissances
Area of application en
Life sciences (including biotechnology)
Area of application fr
Sciences de la vie (y compris biotechnologie)
Research subject code
5201
Research subject group en
Microbiology
Research subject group fr
Microbiologie
Research subject en
Bacteriology
Research subject fr
Bactériologie
Partie
1
Nb partie
1
Application title
Real time PCR for gene expression studies
Keyword
NA
Application summary
The requested real time RT-PCR will allow us to perform gene expression studies in bacteria, nematodes, plants, and mammalian cell lines. This equipment is essential for the research of six different research programs, and will also serve as a valuable tool for other research groups at the university.
The Kumar group is investigating mechanisms that regulate the expression of Resistance-Nodulation-cell Division (RND) family efflux pumps in Acinetobacter baumannii. Analyzing expression of RND operons in different A. baumannii isolates will provide valuable information on how these organisms survive in toxic environments aid in designing new drug therapy against this organism. The Forrester laboratory will use the real time RT-PCR to determine the level of expression of Ligand-Gated Chloride Channels (LGCC) genes in different developmental stages of the parasitic nematodes with a long-term objective of developing anti-helminthics. The Bonetta laboratory will utilize the requested equipment to identify mechanisms important for cell wall synthesis by using a genetic approach in model plant Arabidopsis thaliana. A real time RT-PCR will allow detection of genes that are expressed at low levels. The Strap group will use real time RT-PCR equipment to monitor and compare gene expression of extracellular metabolites produced by bacteria in natural and laboratory conditions. Use of the real time RT-PCR instrument by the Desaulniers group will allow for monitoring of the effect of siRNA molecules on the expression of a variety of genes. The Green-Johnson group will use the real time RT-PCR to examine the ability of functional food components to influence the expression of genes encoding cytokines, key cell surface molecules, and eicosanoids.
The requested equipment will be used by six different research programs that are training a large numbers of Highly Qualified Personnels (HQPs) and therefore will become an extremely useful aspect of HQP training at our institution.
