Grant: CCI Applied Research and Development Grants
Lambton College of Applied Arts and Technology
$38,300.00
Production strain and feedstock validation and scale up of Pichia Pastoris?for Collagen Production
FY 2023-24
Summary
Liven Proteins is a precision fermentation company developing animal-free protein ingredients, such as collagen, with comparable textures and functional properties as animal proteins. Collagen is one of the most abundant proteins produced in the human body, giving stability and strength to various tissues such as tendons, skin, and teeth. Liven Proteins has developed modified strains of Pichia pastoris for collagen production. This yeast is one of the most useful and versatile systems for heterologous protein expression. It is of particular industrial interest due to its tightly regulated methanol-inducible alcohol oxidase 1 promoter (pAOX1), capacity for foreign protein secretion, post-translational modifications capabilities, and ability to grow on defined media at high cell densities, and its strong preference for respiratory.
In collaboration with Lambton College's Bioindustrial Process Research Centre (BPRC), Liven Proteins has proven their proprietary strain produces collagen at high titer. This collaborative research project will focus on the commercialization of biotechnological collagen. Lambton's BPRC will help to improve the bioprocess by testing different raw materials, fermentation protocols, and downstream processes to produce animal-free collagen.
The project's success will allow Liven Proteins to scale up production to 100 L and 1000 L by the end of 2023. This will enable Liven to produce kg-scale samples to provide to their customers in the functional food and beverage companies for testing incorporating in their formulations, thereby helping Liven to secure strategic partners and early customers.
Program
CCI Applied Research and Development Grants
Location
Ontario, Canada
Recipient Type
Academia
Type
Grant
Source
NSERC's Awards Data
As published
Application id
CCARD-2022-00791
Name nom
Sheikhzadeh, Mehdi
Department département
Applied Research and Innovation - Lambton College (Lambton College of Applied Arts and Technology)
Organization id
26621
Institution établissement
Lambton College of Applied Arts and Technology
Province en
Ontario
Province fr
Ontario
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2023
Competition year année de concours
2022
Award amount
38300
Program id
CCARD
Program name en
CCI Applied Research and Development Grants
Program name fr
ICC Subventions de recherche et développement appliquée
Group en
RESEARCH PARTNERSHIPS
Group fr
PARTENARIATS DE RECHERCHE
Area of application code
10000
Area of application group en
The socioeconomic objective available
Area of application group fr
L'objectif socioéconomique disponible
Area of application en
The socioeconomic objective available
Area of application fr
L'objectif socioéconomique disponible
Research subject code
10000
Research subject group en
The field of research available
Research subject group fr
The field of research available
Research subject en
Le domaine de recherche disponible
Research subject fr
Le domaine de recherche disponible
Field of research list codes
RDF2100103
Field of research list names en
Fermentation biotechnology
Field of research list names fr
Biotechnologie de la fermentation
Socioeconomic objective list codes
RDS10602
Socioeconomic objective list names en
Industrial processes related to agricultural products (except wood, paper and fiber)
Socioeconomic objective list names fr
Procédés industriels relatifs aux produits agricoles (sauf le bois, le papier, et la fibre)
Application title
Production strain and feedstock validation and scale up of Pichia Pastoris?for Collagen Production
Keyword
Collagen; Biotechnological Production; Fermentation; Downstream Processing; Fermentation Conditions; Pichia Pastoris Strain; Plant-Based Food; Scale Up
Application summary
Liven Proteins is a precision fermentation company developing animal-free protein ingredients, such as collagen, with comparable textures and functional properties as animal proteins. Collagen is one of the most abundant proteins produced in the human body, giving stability and strength to various tissues such as tendons, skin, and teeth. Liven Proteins has developed modified strains of Pichia pastoris for collagen production. This yeast is one of the most useful and versatile systems for heterologous protein expression. It is of particular industrial interest due to its tightly regulated methanol-inducible alcohol oxidase 1 promoter (pAOX1), capacity for foreign protein secretion, post-translational modifications capabilities, and ability to grow on defined media at high cell densities, and its strong preference for respiratory.
In collaboration with Lambton College's Bioindustrial Process Research Centre (BPRC), Liven Proteins has proven their proprietary strain produces collagen at high titer. This collaborative research project will focus on the commercialization of biotechnological collagen. Lambton's BPRC will help to improve the bioprocess by testing different raw materials, fermentation protocols, and downstream processes to produce animal-free collagen.
The project's success will allow Liven Proteins to scale up production to 100 L and 1000 L by the end of 2023. This will enable Liven to produce kg-scale samples to provide to their customers in the functional food and beverage companies for testing incorporating in their formulations, thereby helping Liven to secure strategic partners and early customers.
