Grant: Discovery Grants Program - Individual

Goal of this work is to develop quantitative measurements in scattering media for both high and low concentration constituents. One aim of the program will provide a better basic understanding of the interaction of scattering in spectroscopic measurements. Quantification of constituents in scattering sample by optical measurement is limited by knowledge of the optical path in the sample. We propose to use a ultrasonically modulated optical approach to gain a knowledge of the scattering properties of a sample and thereby, improve quantification of optically absorbing constituents. The basic information gained in this work will provide approaches useful in fields ranging from biofluid analysis to pharmacueticals and paint manufacturing.The other aim of the program will be development and characterization of a new class of ultrasound sensitive probes for low concentration analyte detection which have wide application in bioanalysis. We are developing nano/micro-probes made from polymer hydrogels fashioned into small structures which undergo volume phase transition. Changes in the micro-probe structure upon analyte binding vary the bulk physical properties. These sensors have broad application for biofluids such as brackish water, cells suspension and blood analysis.This work will provide the fundamental understanding of the dynamic range of use of the sensors and the linear and non-linear ultrasonic properties which govern them. In the future, methodologies developed in this project provides the basis for non-invasive and minimally invasive quantification for routine research and clinical use.