Grant: Research Tools and Instruments - Category 1 (<$150,000)
Université du Québec à Montréal
$54,174.00
A protein purification platform for biochemistry and structural biology research.
FY 2012-13
Summary
The present proposal is prepared to request a complete system to perform fast protein liquid chromatography (FPLC) in oder to establish a complete platform for protein expression and purifcation at UQ?M. FPLC is an experimental technique that allows the very efficient separation, in non-denaturing and low pressure condition, of a complex ensemble of biomolecules in order to purify, characterize, quantify and identify a particular compound of the initial mixture, usually proteins, protein-complexes and nucleic acids. Briefly, this liquid chromatographic technique is supported by the elution of a mobile phase (analyte) through a stationary phase (column) following by the analysis with a detector. This equipment will play a central role for the achievement of our research programs, as we need highly-pure and well-characterized protein preparation for our biological, biochemical or structural investigations. Several research programs that are underway at the Department will be supported by this apparatus. For instance, we will use this FPLC to isolate soluble protein aggregates that are precursors of amyloid fibrils associated with various diseases for (bio)chemical and biological studies. Besides, this system will support the structural study by NMR of the drug-induced long QT syndrome by allowing the purification of fragments of the ether-à-gogo-related gene (hERG) potassium channel that will be previously expressed in bacteria. Other research projects that will be supported with this infrastructure include the understanding of the structure-to-function impact of the glucose-6-phosphate translocase G6PT, the elucidation of the function of ceruloplasmin in the nervous system development and the study of the cardioprotective potential of copper-containing amine-oxidase enzymes. The research projects that will be supported with this requested FPLC will allow a better understanding of the molecular basis of physiological processes in normal and/or disease state and could ultimately lead to innovative therapeutic approaches for different diseases, such as cancer, the drug-induced long QT syndrome, protein misfolding disorders and developmental disorders associated with neuronal disorganization.
Program
Research Tools and Instruments - Category 1 (<$150,000)
Recipient
Location
Quebec, Canada
Recipient Type
Academia
Type
Grant
Source
NSERC's Awards Data
As published
Application id
440105-2013
Name nom
Bourgault, Steve
Department département
Chimie
Organization id
57
Institution établissement
Université du Québec à Montréal
Province en
Québec
Province fr
Québec
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2012
Competition year année de concours
2013
Award amount
54174
Program id
EQPEQ
Program name en
Research Tools and Instruments - Category 1 (<$150,000)
Program name fr
Outils et instruments de recherche - catégorie 1 (<150 000$)
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
1501
Committee name en
Genes, Cells and Molecules
Committee name fr
Gènes, cellules et molécules
Area of application code
1201
Area of application group en
Advancement of knowledge
Area of application group fr
Avancement des connaissances
Area of application en
Life sciences (including biotechnology)
Area of application fr
Sciences de la vie (y compris biotechnologie)
Research subject code
5400
Research subject group en
Biochemistry
Research subject group fr
Biochimie
Research subject en
Biochemistry
Research subject fr
Biochimie
Partie
1
Nb partie
1
Application title
A protein purification platform for biochemistry and structural biology research.
Keyword
NSERC
Application summary
The present proposal is prepared to request a complete system to perform fast protein liquid chromatography (FPLC) in oder to establish a complete platform for protein expression and purifcation at UQ?M. FPLC is an experimental technique that allows the very efficient separation, in non-denaturing and low pressure condition, of a complex ensemble of biomolecules in order to purify, characterize, quantify and identify a particular compound of the initial mixture, usually proteins, protein-complexes and nucleic acids. Briefly, this liquid chromatographic technique is supported by the elution of a mobile phase (analyte) through a stationary phase (column) following by the analysis with a detector. This equipment will play a central role for the achievement of our research programs, as we need highly-pure and well-characterized protein preparation for our biological, biochemical or structural investigations. Several research programs that are underway at the Department will be supported by this apparatus. For instance, we will use this FPLC to isolate soluble protein aggregates that are precursors of amyloid fibrils associated with various diseases for (bio)chemical and biological studies. Besides, this system will support the structural study by NMR of the drug-induced long QT syndrome by allowing the purification of fragments of the ether-à-gogo-related gene (hERG) potassium channel that will be previously expressed in bacteria. Other research projects that will be supported with this infrastructure include the understanding of the structure-to-function impact of the glucose-6-phosphate translocase G6PT, the elucidation of the function of ceruloplasmin in the nervous system development and the study of the cardioprotective potential of copper-containing amine-oxidase enzymes. The research projects that will be supported with this requested FPLC will allow a better understanding of the molecular basis of physiological processes in normal and/or disease state and could ultimately lead to innovative therapeutic approaches for different diseases, such as cancer, the drug-induced long QT syndrome, protein misfolding disorders and developmental disorders associated with neuronal disorganization.
