Grant: Discovery Grants Program - Individual
Université de Sherbrooke
$64,000.00
Biochemical and Genetic Characterization of Ribosome Biogenesis and Functional Diversity
FY 2020-21
Summary
Ribosomes
are intricate molecular machines that play a central role in translating the
genetic code into proteins. In baker's yeast, assembly of the ribosomal RNA
requires a large number of factors and the correct expression of 137 ribosomal
protein-coding genes the majority of which are duplicated genes. The events
leading to the processing of pre-rRNAs, assembly and export have been
extensively studied in vertebrates and yeast, and the overall process is very
well understood. However, the mechanism regulating the expression of duplicated
ribosomal protein genes and its impact on ribosome biogenesis and function
remain unclear. Recently, we discovered that the majority of the ribosomal
protein paralogs are not expressed equally and that in most cases they are
expressed in a specific ratio required for normal growth. This finding argues
against an equal and redundant role for the ribosomal protein genes paralogs. In
this proposal, we aim at understanding the mechanism regulating the expression
of duplicated genes and its impact on ribosome production and function. Accordingly,
we propose two specific aims: 1. Determine the mechanism regulating the
expression of duplicated ribosomal protein genes. Under this aim, we will
determine how duplicated genes communicate to establish the overall expression
of ribosomal proteins. We have recently identified a set of inter-regulated
duplicated ribosomal genes, and we will use them as a model for this study. The
impact of promoters, intron, transcription termination and cellular
ribonucleases on the expression of these paralogs will be monitored and
elements that affect inter and intragene regulation will be identified. The
outcome of this study will not only explain how the expression of ribosomal
proteins is coordinated but will also help us to understand an important
component of the general mechanism of gene regulation. 2. Understand the
impact of duplicated genes on ribosome biogenesis and function. Under this
aim, we will monitor the impact of changing the ratio of ribosomal proteins on
ribos
Program
Discovery Grants Program - Individual
Recipient
Location
Quebec, Canada
Recipient Type
Academia
Type
Grant
Source
NSERC's Awards Data
As published
Application id
RGPIN-2016-03729
Name nom
Abou Elela, Sherif
Department département
Microbiology
Organization id
68
Institution établissement
Université de Sherbrooke
Province en
Québec
Province fr
Québec
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2020
Competition year année de concours
2016
Award amount
64000
Program id
RGPIN
Program name en
Discovery Grants Program - Individual
Program name fr
Programme de subventions à la découverte - individuelles
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
1501
Committee name en
Genes, Cells and Molecules
Committee name fr
Gènes, cellules et molécules
Area of application code
1201
Area of application group en
Advancement of knowledge
Area of application group fr
Avancement des connaissances
Area of application en
Life sciences (including biotechnology)
Area of application fr
Sciences de la vie (y compris biotechnologie)
Research subject code
5303
Research subject group en
Molecular biology
Research subject group fr
Biologie moléculaire
Research subject en
Gene regulation
Research subject fr
Régulation des gènes
Installment
inst:5-6
Partie
2
Nb partie
6
Application title
Biochemical and Genetic Characterization of Ribosome Biogenesis and Functional Diversity
Keyword
NSERC
Application summary
Ribosomes
are intricate molecular machines that play a central role in translating the
genetic code into proteins. In baker's yeast, assembly of the ribosomal RNA
requires a large number of factors and the correct expression of 137 ribosomal
protein-coding genes the majority of which are duplicated genes. The events
leading to the processing of pre-rRNAs, assembly and export have been
extensively studied in vertebrates and yeast, and the overall process is very
well understood. However, the mechanism regulating the expression of duplicated
ribosomal protein genes and its impact on ribosome biogenesis and function
remain unclear. Recently, we discovered that the majority of the ribosomal
protein paralogs are not expressed equally and that in most cases they are
expressed in a specific ratio required for normal growth. This finding argues
against an equal and redundant role for the ribosomal protein genes paralogs. In
this proposal, we aim at understanding the mechanism regulating the expression
of duplicated genes and its impact on ribosome production and function. Accordingly,
we propose two specific aims: 1. Determine the mechanism regulating the
expression of duplicated ribosomal protein genes. Under this aim, we will
determine how duplicated genes communicate to establish the overall expression
of ribosomal proteins. We have recently identified a set of inter-regulated
duplicated ribosomal genes, and we will use them as a model for this study. The
impact of promoters, intron, transcription termination and cellular
ribonucleases on the expression of these paralogs will be monitored and
elements that affect inter and intragene regulation will be identified. The
outcome of this study will not only explain how the expression of ribosomal
proteins is coordinated but will also help us to understand an important
component of the general mechanism of gene regulation. 2. Understand the
impact of duplicated genes on ribosome biogenesis and function. Under this
aim, we will monitor the impact of changing the ratio of ribosomal proteins on
ribos
