Grant: CIHR Fellowship

Cystic fibrosis (CF) is the most common lethal genetic disease in Canada, profoundly affecting the respiratory system. This condition arises from mutations in a gene that lead to the production of thick mucus in the lungs. This thick mucus creates an environment that is highly prone to chronic infections, often resulting in significant lung damage and respiratory failure. A key player in this scenario is the bacterium Pseudomonas aeruginosa, which can adapt to the CF environment and worsen lung health. Despite advancements in treatment options, CF remains a complex condition, and our understanding of how bacteria interact with lung cells continues to evolve. Recent studies indicate that the types of bacteria present in the lungs can vary considerably based on the affected lung region. This project aims to investigate these localized interactions between bacteria and lung cells, focusing on how they contribute to disease progression and affect the immune response. Our research has three main objectives: 1.Characterizing Bacterial Populations: We will develop and apply innovative methods to study the diverse bacterial strains present in different regions of CF lungs by analyzing lung tissue samples from patients. 2.Investigating Cell Responses: We will enhance techniques to examine how lung cells respond at the genetic level to these bacterial populations, deepening our understanding of their reactions to infections. 3.Mapping Host-Bacteria Interactions: Utilizing cutting-edge technologies, we will visualize how bacteria and lung cells interact in specific areas, shedding light on localized effects and the progression of the disease. Through this research, we aim to clarify the complex relationships between bacteria and lung health in CF. Ultimately, our findings could pave the way for more targeted treatments and improved outcomes for patients by identifying new biomarkers for assessing lung damage and infection.