Grant: Research Tools and Instruments - Category 1 (<$150,000)
Queen's University
$149,911.00
Flow Cytometry Cell Sorting Analysis Research Platform.
FY 2014-15
Summary
Fluorescence Activated Cell Sorting (FACS) is routinely used to enrich for a specific cell type from a heterogeneous cell suspension. FACS sorting relies on the binding of fluorescence-tagged antibodies to specific biomarkers such as receptors on the surface of the cell. The characteristic fluorescent properties activated by laser emissions readily identify cells of interest and permits their separation at exceptional purity. This is an effective and rapid approach to isolate many biological cell types and will catalyze much deeper levels of our ongoing biological research that are aimed at integrating intracellular signaling events with functions in the specific cell types of interest. The dual laser Sony SH800 high-speed FACS with a biosafety cabinet is requested to support NSERC-funded research work in the fields of and cell biology, genetics, biotechnology, infection, immunity, and inflammation. This equipment will sort cells that require biohazard level II containment, a feature not currently available at Queen's University. The biosafety feature is essential to limit the exposure of highly qualified personnel (HQP) to infectious agents and to maintain the integrity of the biological samples for transplantation or culture following collection.Cell sorting underpins research activities in many of our basic research laboratories that are NSERC-funded. This instrument is essential for the success of the applicants' NSERC-funded research projects and this gap in capability to sort cells at Biohazard level II is recognized as a strategic priority for correction by the leadership of the Faculty of Health Sciences. This proposal benefits considerably from Faculty support of a substantial cash contribution towards purchase of this instrument. This will ensure our ability to improve training for our HQP and will enhance our ability to produce research discoveries at the next frontier. It will also ensure that the co-applicants remain nationally and internationally competitive in their research fields.
Program
Research Tools and Instruments - Category 1 (<$150,000)
Recipient
Location
Ontario, Canada
Recipient Type
Academia
Type
Grant
Source
NSERC's Awards Data
As published
Application id
472604-2015
Name nom
Basta, Sameh
Department département
Biomedical and Molecular Sciences
Organization id
29
Institution établissement
Queen's University
Province en
Ontario
Province fr
Ontario
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2014
Competition year année de concours
2015
Award amount
149911
Program id
EQPEQ
Program name en
Research Tools and Instruments - Category 1 (<$150,000)
Program name fr
Outils et instruments de recherche - catégorie 1 (<150 000$)
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
1501
Committee name en
Genes, Cells and Molecules
Committee name fr
Gènes, cellules et molécules
Area of application code
1201
Area of application group en
Advancement of knowledge
Area of application group fr
Avancement des connaissances
Area of application en
Life sciences (including biotechnology)
Area of application fr
Sciences de la vie (y compris biotechnologie)
Research subject code
5100
Research subject group en
Cell biology
Research subject group fr
Biologie cellulaire
Research subject en
Cell biology
Research subject fr
Biologie cellulaire
Partie
1
Nb partie
1
Application title
Flow Cytometry Cell Sorting Analysis Research Platform.
Keyword
NSERC
Application summary
Fluorescence Activated Cell Sorting (FACS) is routinely used to enrich for a specific cell type from a heterogeneous cell suspension. FACS sorting relies on the binding of fluorescence-tagged antibodies to specific biomarkers such as receptors on the surface of the cell. The characteristic fluorescent properties activated by laser emissions readily identify cells of interest and permits their separation at exceptional purity. This is an effective and rapid approach to isolate many biological cell types and will catalyze much deeper levels of our ongoing biological research that are aimed at integrating intracellular signaling events with functions in the specific cell types of interest. The dual laser Sony SH800 high-speed FACS with a biosafety cabinet is requested to support NSERC-funded research work in the fields of and cell biology, genetics, biotechnology, infection, immunity, and inflammation. This equipment will sort cells that require biohazard level II containment, a feature not currently available at Queen's University. The biosafety feature is essential to limit the exposure of highly qualified personnel (HQP) to infectious agents and to maintain the integrity of the biological samples for transplantation or culture following collection.Cell sorting underpins research activities in many of our basic research laboratories that are NSERC-funded. This instrument is essential for the success of the applicants' NSERC-funded research projects and this gap in capability to sort cells at Biohazard level II is recognized as a strategic priority for correction by the leadership of the Faculty of Health Sciences. This proposal benefits considerably from Faculty support of a substantial cash contribution towards purchase of this instrument. This will ensure our ability to improve training for our HQP and will enhance our ability to produce research discoveries at the next frontier. It will also ensure that the co-applicants remain nationally and internationally competitive in their research fields.
