Grant: Research Tools and Instruments - Category 1 (<$150,000)
University of Ontario Institute of Technology
$75,000.00
High performance liquid chromatography (HPLC) for interdisciplinary research
FY 2009-10
Summary
A high performance liquid chromatography (HPLC) system will allow interdisciplinary research groups at UOIT to analyze and purify a range of compounds including low molecular weight signaling molecules, bacterial metabolites, peptides, oligonucleotides and small interfering RNAs (siRNAs). The equipment is essential for the research programs of four research groups, and will provide opportunity for training graduate students and highly qualified research personnel within the broader UOIT research community. The Strap research group has been working to identify key signaling molecules involved in plant-microbe interactions as well as the microbial contribution to biomass turnover. This research will have a positive impact on Canadian agriculture and will aid in the development of improved cellulosic feedstock for use in biosolvent production. In addition, the Strap research group in collaboration with the Kumar research group has identified and partially purified novel, bioactive microbial compounds effective against a broad spectrum of human pathogens. The acquisition of an HPLC system is necessary to purify these compounds for structural determination. This research will significantly impact Canadian society by providing new knowledge about microbial resistance mechanisms and will allow for identification of new therapeutic targets to deal with highly resistant pathogens. The Bonetta research group aims to understand mechanisms that govern cell wall synthesis and assembly in plants. An HPLC will allow characterization of cell wall mutants through determination of oligosaccharide profiles of wall material. The insights gained from this research will be crucial to the development of cell wall derived products such as plastics, fibres, and fuels, which have the potential to improve the sustainability of Canada's natural resources and environmental quality. The Desaulniers research group is investigating the effect of novel chemical modifications on stability and efficacy of siRNA to develop therapeutic agents to treat infections. HPLC is necessary for purification of newly synthesized modified siRNAs for subsequent structure-function experiments.
Program
Research Tools and Instruments - Category 1 (<$150,000)
Location
Ontario, Canada
Recipient Type
Academia
Type
Grant
Source
NSERC's Awards Data
As published
Application id
391097-2010
Name nom
Strap, Janice
Department département
Science, Faculty of
Organization id
19222
Institution établissement
University of Ontario Institute of Technology
Province en
Ontario
Province fr
Ontario
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2009
Competition year année de concours
2010
Award amount
75000
Program id
EQPEQ
Program nane en
Research Tools and Instruments - Category 1 (<$150,000)
Program name fr
Outils et instruments de recherche - catégorie 1 (<150 000$)
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
1501
Committee name en
Genes, Cells and Molecules
Committee name fr
Gènes, cellules et molécules
Area of application code
1201
Area of application group en
Advancement of knowledge
Area of application group fr
Avancement des connaissances
Area of application en
Life sciences (including biotechnology)
Area of application fr
Sciences de la vie (y compris biotechnologie)
Research subject code
5200
Research subject group en
Microbiology
Research subject group fr
Microbiologie
Research subject en
Microbiology
Research subject fr
Microbiologie
Partie
1
Nb partie
1
Application title
High performance liquid chromatography (HPLC) for interdisciplinary research
Keyword
NA
Application summary
A high performance liquid chromatography (HPLC) system will allow interdisciplinary research groups at UOIT to analyze and purify a range of compounds including low molecular weight signaling molecules, bacterial metabolites, peptides, oligonucleotides and small interfering RNAs (siRNAs). The equipment is essential for the research programs of four research groups, and will provide opportunity for training graduate students and highly qualified research personnel within the broader UOIT research community. The Strap research group has been working to identify key signaling molecules involved in plant-microbe interactions as well as the microbial contribution to biomass turnover. This research will have a positive impact on Canadian agriculture and will aid in the development of improved cellulosic feedstock for use in biosolvent production. In addition, the Strap research group in collaboration with the Kumar research group has identified and partially purified novel, bioactive microbial compounds effective against a broad spectrum of human pathogens. The acquisition of an HPLC system is necessary to purify these compounds for structural determination. This research will significantly impact Canadian society by providing new knowledge about microbial resistance mechanisms and will allow for identification of new therapeutic targets to deal with highly resistant pathogens. The Bonetta research group aims to understand mechanisms that govern cell wall synthesis and assembly in plants. An HPLC will allow characterization of cell wall mutants through determination of oligosaccharide profiles of wall material. The insights gained from this research will be crucial to the development of cell wall derived products such as plastics, fibres, and fuels, which have the potential to improve the sustainability of Canada's natural resources and environmental quality. The Desaulniers research group is investigating the effect of novel chemical modifications on stability and efficacy of siRNA to develop therapeutic agents to treat infections. HPLC is necessary for purification of newly synthesized modified siRNAs for subsequent structure-function experiments.
