Grant: Research Tools and Instruments
York University
$149,827.00
Flow Cytometer for Muscle Health Research
FY 2019-20
Summary
******Flow Cytometry is a method which allows the researcher to examine the biochemical properties of individual cells, or organelles derived from cells, using fluorescent labeling techniques. It relies on the use of lasers which produce different wavelengths which can be used to excite and detect molecules within those cells or organelles. In this application, I am requesting funds to acquire only the third such equipment system on the entire York campus, and to replace my obsolete system which served us well in the past, but which now costs more to maintain than to use, and is now not operational. My trainees and colleagues will continue to use flow cytometry to analyze the biochemical properties of muscle cells in their immature (Myoblasts) and mature state (myotubes), as well as the characteristics of skin fibroblasts from healthy controls as well as mitochondrial myopathy patients. We also use flow cytometry to analyze the function of isolated mitochondria and mitochondrial phospholipids (eg. Cardiolipin) from healthy and exercised animal models, to support to support our NSERC Discovery grant objectives. Acquisition of this new equipment is absolutely essential for us to progress in achieving our NSERC DG research goals. A delay in its acquisition will have a significant impact on our progress, since we are waiting for a replacement. In addition, this equipment will have a significant impact on the training of HQP. My laboratory is in high demand for MSc, PhD and post-doctoral opportunities, and I currently have 12 trainees on a yearly basis. The flow cytometry system will allow me to train these HQP in marketable, sophisticated and up-to-date technology, which will serve them well in academic and other research and analysis settings. In addition, as the Director of the Muscle Health Research Centre (MHRC), I am also submitting this application to help support the >30 other faculty members in the Department of Biology, the School of Kinesiology and Health Science, and the MHRC, with the goal of increasing our currently very limited capacity for cellular analyses using this important methodology at York University. **
Program
Research Tools and Instruments
Recipient
Location
Ontario, Canada
Recipient Type
Academia
Type
Grant
Source
NSERC's Awards Data
As published
Application id
RTI-2020-00599
Name nom
Hood, David
Department département
Kinesiology and Health Science, School of
Organization id
38
Institution établissement
York University
Province en
Ontario
Province fr
Ontario
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2019
Competition year année de concours
2020
Award amount
149827
Program id
RTI
Program name en
Research Tools and Instruments
Program name fr
Outils et instruments de recherche
Group en
DISCOVERY RESEARCH
Group fr
RECHERCHE AXÉE SUR LA DÉCOUVERTE
Committee code
1602
Committee name en
Biological Systems and Functions (RTI)
Committee name fr
Systèmes et fonctions biologiques (OIR)
Area of application code
1101
Area of application group en
Health, education and social services
Area of application group fr
Santé publique, éducation et services sociaux
Area of application en
Human health (including medically-related psychological research)
Area of application fr
Santé publique (y compris recherche psychologique liée à la médecine)
Research subject code
5100
Research subject group en
Cell biology
Research subject group fr
Biologie cellulaire
Research subject en
Cell biology
Research subject fr
Biologie cellulaire
Installment
inst:1 - 1
Partie
1
Nb partie
1
Application title
Flow Cytometer for Muscle Health Research
Keyword
NSERC
Application summary
******Flow Cytometry is a method which allows the researcher to examine the biochemical properties of individual cells, or organelles derived from cells, using fluorescent labeling techniques. It relies on the use of lasers which produce different wavelengths which can be used to excite and detect molecules within those cells or organelles. In this application, I am requesting funds to acquire only the third such equipment system on the entire York campus, and to replace my obsolete system which served us well in the past, but which now costs more to maintain than to use, and is now not operational. My trainees and colleagues will continue to use flow cytometry to analyze the biochemical properties of muscle cells in their immature (Myoblasts) and mature state (myotubes), as well as the characteristics of skin fibroblasts from healthy controls as well as mitochondrial myopathy patients. We also use flow cytometry to analyze the function of isolated mitochondria and mitochondrial phospholipids (eg. Cardiolipin) from healthy and exercised animal models, to support to support our NSERC Discovery grant objectives. Acquisition of this new equipment is absolutely essential for us to progress in achieving our NSERC DG research goals. A delay in its acquisition will have a significant impact on our progress, since we are waiting for a replacement. In addition, this equipment will have a significant impact on the training of HQP. My laboratory is in high demand for MSc, PhD and post-doctoral opportunities, and I currently have 12 trainees on a yearly basis. The flow cytometry system will allow me to train these HQP in marketable, sophisticated and up-to-date technology, which will serve them well in academic and other research and analysis settings. In addition, as the Director of the Muscle Health Research Centre (MHRC), I am also submitting this application to help support the >30 other faculty members in the Department of Biology, the School of Kinesiology and Health Science, and the MHRC, with the goal of increasing our currently very limited capacity for cellular analyses using this important methodology at York University. **
