Grant: CIHR Fellowship
Direct Payment
$150,000.00
Selection and capture of foreign DNA by type II CRISPR-Cas systems
FY 2016-17
Summary
The study of CRISPR-Cas, a bacterial system that defends against viruses and other foreign DNA, has led to a huge breakthrough in biotechnology: CRISPR-Cas9 genome editing. By understanding the mechanism by which the bacterial enzyme, Cas9, uses an RNA guide sequence to target specific DNA sequences for cleavage, researchers including those in the Doudna lab have developed a powerful technology to alter DNA sequences in living human cells. Here, I propose to study a different, poorly understood stage of CRISPR- Cas immunity: how bacteria select and capture a genetic "memory" of invading DNA for later deployment as RNA guides for Cas9. The results from my project will uncover the molecular mechanisms by which bacteria distinguish self from non-self DNA. Moreover, the integration of DNA segments into a defined genomic locus could be used for several applications, including lineage tracing of individual eukaryotic cells, and heritable recording of chronological molecular events.
Department
Program
CIHR Fellowship
Recipient
Location
California, United States of America
Type
Grant
Source
CIHR Funding Decisions Database
As published
Id
361790
Name
Pawluk April
Region
California
Country
United States of America
Orgtype
Research Institution
Instname2
Genetics
Theme2
Biomedical
Prcname2
Fellowships - Post-PhD
Projecttitle
Selection and capture of foreign DNA by type II CRISPR-Cas systems
Programtype2
Fellowship Programs
Orgnameinp2
Direct Payment
Cihramount2
$150,000
Cihrequipment2
$0
Programname2
CIHR Fellowship
Keyworddelim
Bacteriophage; Cas9; Crispr-Cas; Nucleic Acid Processing; Protein-Protein Interactions; Streptococcus Pyogenes
Abstract
The study of CRISPR-Cas, a bacterial system that defends against viruses and other foreign DNA, has led to a huge breakthrough in biotechnology: CRISPR-Cas9 genome editing. By understanding the mechanism by which the bacterial enzyme, Cas9, uses an RNA guide sequence to target specific DNA sequences for cleavage, researchers including those in the Doudna lab have developed a powerful technology to alter DNA sequences in living human cells. Here, I propose to study a different, poorly understood stage of CRISPR- Cas immunity: how bacteria select and capture a genetic "memory" of invading DNA for later deployment as RNA guides for Cas9. The results from my project will uncover the molecular mechanisms by which bacteria distinguish self from non-self DNA. Moreover, the integration of DNA segments into a defined genomic locus could be used for several applications, including lineage tracing of individual eukaryotic cells, and heritable recording of chronological molecular events.
Competitiondate
201611
Approvedterm2
3 yrs 0 mth
Pinamesdelim
Pawluk, April
Approvedterm
3 ans 0 mois
Cihrcontribution2
~ $150,000~ $0~
