Grant: Research Tools and Instruments

Gene expression regulation directly impacts many physiological processes such as development, reproduction, endocrinology and metabolism. This proposal is for the acquisition of a comprehensive system made of a standard thermocycler for cDNA synthesis and a highly sensitive real-time quantitative PCR (qPCR) for gene expression quantification from these cDNA samples. The requested equipment is essential to investigate the regulatory mechanisms of gene expression in different types of cells and animal models. In addition, the requested equipment will be used for genomic DNA amplification, evaluation of protein-DNA interactions, as well as molecular cloning and sequencing library preparation. With this system, quantification of gene expression will be supported in several laboratories to address different research questions related to transcriptional regulation, non-coding RNAs and epigenetics. The training and education of highly qualified personnel in an equitable, diversified, and inclusive environment will also be promoted by the use of the requested equipment. In this way, each mentored student will receive essential and practical training related to a wide range of analyses using this comprehensive system. Thus, students will acquire valuable and transferable skills, and benefit from key opportunities to advance their respective research projects and future careers.