Grant: Research Tools and Instruments - Category 1 (<$150,000)
Université du Québec à Montréal
$75,233.00
Liquid chromatography for ultra-high performance detection of glutathione and phytochelatins involved in the sequestration and detoxification of pollutants
FY 2012-13
Summary
In this research program, phytochelatins, glutathione and related thiol-peptides involved in pollutants cellular sequestration and detoxification mechanisms will be investigated with ultra-high-performance liquid chromatography. This research instrument is composed of a core system permitting high-efficient separation of these proteins through specialized columns, an automated sample injection for high-throughput analysis, and a fluorescence detector providing high-sensitive qualitative and quantitative detection of these proteins. This analytical approach will be used on green algae strains exposed to different concentrations of aquatic pollutants to identify the different biochemical properties of algae related to the transport and sequestration of aquatic pollutants in the cell vacuole. The results obtained by this approach will be used to develop a fundamental model which identifies the best algal strain characteristics to obtain the most efficient vacuole sequestration of pollutants. This model will be used to develop new biotechnological approaches to remediate contaminated wastewater. This environmental biotechnology using algal detoxification mechanisms can be applied to prevent the pollution of aquatic ecosystems and protect the quality of the environment.
Program
Research Tools and Instruments - Category 1 (<$150,000)
Recipient
Location
Quebec, Canada
Recipient Type
Academia
Type
Grant
Source
NSERC's Awards Data
As published
Application id
440076-2013
Name nom
Dewez, David
Department département
Chimie
Organization id
57
Institution établissement
Université du Québec à Montréal
Province en
Québec
Province fr
Québec
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2012
Competition year année de concours
2013
Award amount
75233
Program id
EQPEQ
Program name en
Research Tools and Instruments - Category 1 (<$150,000)
Program name fr
Outils et instruments de recherche - catégorie 1 (<150 000$)
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
1501
Committee name en
Genes, Cells and Molecules
Committee name fr
Gènes, cellules et molécules
Area of application code
406
Area of application group en
Environment
Area of application group fr
Environnement
Area of application en
Pollutants and toxic agents (waste, use 902)
Area of application fr
Polluants et agents toxiques (déchets, utiliser 902)
Research subject code
5400
Research subject group en
Biochemistry
Research subject group fr
Biochimie
Research subject en
Biochemistry
Research subject fr
Biochimie
Partie
1
Nb partie
1
Application title
Liquid chromatography for ultra-high performance detection of glutathione and phytochelatins involved in the sequestration and detoxification of pollutants
Keyword
NSERC
Application summary
In this research program, phytochelatins, glutathione and related thiol-peptides involved in pollutants cellular sequestration and detoxification mechanisms will be investigated with ultra-high-performance liquid chromatography. This research instrument is composed of a core system permitting high-efficient separation of these proteins through specialized columns, an automated sample injection for high-throughput analysis, and a fluorescence detector providing high-sensitive qualitative and quantitative detection of these proteins. This analytical approach will be used on green algae strains exposed to different concentrations of aquatic pollutants to identify the different biochemical properties of algae related to the transport and sequestration of aquatic pollutants in the cell vacuole. The results obtained by this approach will be used to develop a fundamental model which identifies the best algal strain characteristics to obtain the most efficient vacuole sequestration of pollutants. This model will be used to develop new biotechnological approaches to remediate contaminated wastewater. This environmental biotechnology using algal detoxification mechanisms can be applied to prevent the pollution of aquatic ecosystems and protect the quality of the environment.
