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Grant: Research Tools and Instruments - Category 1 (<$150,000)

Memorial University of Newfoundland

$90,029.00

Upgrade and expansion of microscopy and imaging suite for multifaceted studies

FY 2014-15

Summary
I am requesting funds for the upgrade and replacement of key microscopy and imaging systems, central to all my research, including my NSERC-funded program on the chronobiology and control of biological processes in marine organisms. New equipment is urgently needed to: (1) re-align our imaging capacities to modern world-class standards that would enable us to remain competitive internationally and retain key collaborations; (2) meet the growing needs of students and other highly qualified personnel; and (3) maximize our research efficiency and originality by making use of the newest technologies in our field. We use brightfield, darkfield, phase-contrast and epi-fluorescence techniques to identify, measure and monitor specialized cells, developing gametes, fertilization events, embryonic and larval attributes, symbiotic associations, and gut contents, as well as to prepare videos and microphotographic plates for publication. My Discovery research program integrates concepts of reproductive biology, chronobiology and chemical ecology to disentangle the respective roles played by biological clocks, external cues and social interactions in reproductive processes. Its principal aim is to gain a finer understanding of benthic ecosystem functioning by identifying the drivers of multileveled biological rhythms in marine taxa, including possible phase shifts triggered by climate change. Microscopic investigations and the proposed tools are also at the core of my other fundamental and applied research foci, such as trophic ecology, species interactions, taxonomy, the biology of deep-sea corals and the effects of stressors (pollutants, ocean acidification) on calcification, lipid storage, growth and reproduction. Over 80% of the publications coming out of my lab depend on microscopy and imaging, and it is expected that the proposed suite of tools will directly support the training of 3 to 8 students/trainees annually. Their findings will not only provide insight into our evolutionary past but also help develop aquaculture techniques and time-scale-based management and conservation strategies.
Program
Research Tools and Instruments - Category 1 (<$150,000)
Location
Newfoundland and Labrador, Canada
Recipient Type
Academia
Type
Grant
Source
NSERC's Awards Data

As published

Application id
472525-2015
Name nom
Mercier, Annie
Department département
Ocean Sciences
Organization id
89
Institution établissement
Memorial University of Newfoundland
Province en
Newfoundland and Labrador
Province fr
Terre-Neuve-et-Labrador
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2014
Competition year année de concours
2015
Award amount
90029
Program id
EQPEQ
Program name en
Research Tools and Instruments - Category 1 (<$150,000)
Program name fr
Outils et instruments de recherche - catégorie 1 (<150 000$)
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
1503
Committee name en
Evolution and Ecology
Committee name fr
Évolution et écologie
Area of application code
1200
Area of application group en
Advancement of knowledge
Area of application group fr
Avancement des connaissances
Area of application en
Advancement of knowledge
Area of application fr
Avancement des connaissances
Research subject code
5500
Research subject group en
Animal biology
Research subject group fr
Biologie animale
Research subject en
Animal biology
Research subject fr
Biologie animale
Partie
1
Nb partie
1
Application title
Upgrade and expansion of microscopy and imaging suite for multifaceted studies
Keyword
NSERC
Application summary
I am requesting funds for the upgrade and replacement of key microscopy and imaging systems, central to all my research, including my NSERC-funded program on the chronobiology and control of biological processes in marine organisms. New equipment is urgently needed to: (1) re-align our imaging capacities to modern world-class standards that would enable us to remain competitive internationally and retain key collaborations; (2) meet the growing needs of students and other highly qualified personnel; and (3) maximize our research efficiency and originality by making use of the newest technologies in our field. We use brightfield, darkfield, phase-contrast and epi-fluorescence techniques to identify, measure and monitor specialized cells, developing gametes, fertilization events, embryonic and larval attributes, symbiotic associations, and gut contents, as well as to prepare videos and microphotographic plates for publication. My Discovery research program integrates concepts of reproductive biology, chronobiology and chemical ecology to disentangle the respective roles played by biological clocks, external cues and social interactions in reproductive processes. Its principal aim is to gain a finer understanding of benthic ecosystem functioning by identifying the drivers of multileveled biological rhythms in marine taxa, including possible phase shifts triggered by climate change. Microscopic investigations and the proposed tools are also at the core of my other fundamental and applied research foci, such as trophic ecology, species interactions, taxonomy, the biology of deep-sea corals and the effects of stressors (pollutants, ocean acidification) on calcification, lipid storage, growth and reproduction. Over 80% of the publications coming out of my lab depend on microscopy and imaging, and it is expected that the proposed suite of tools will directly support the training of 3 to 8 students/trainees annually. Their findings will not only provide insight into our evolutionary past but also help develop aquaculture techniques and time-scale-based management and conservation strategies.
Memorial University of Newfoundland - $90,029 Grant (… | Canada Spends