Grant: Research Tools and Instruments - Category 1 (<$150,000)
Carleton University
$61,752.00
A visualized patch clamp recording system
EF 2006-07
Résumé
A visualized patch clamp recording system is needed to extend my current program of research on the central auditory system (NSERC Discovery Grant supported). The equipment will allow us to carry out the investigation of cellular physiology of the auditory midbrain. The visualized patch clamp recording method has the advantage over blind recordings of allowing detailed investigation of ion channels and improved resolution of physiological events underlying signal processing. The visualized patch clamp recording method is especially necessary for studying developing neurons, such as those in the auditory brainstem of young animals. These neurons are too small or fragile to impale using a sharp electrode recording or a blind patch clamp method. Stable recordings will be achieved with a visualized patch clamp recording system. It is then possible to apply various pharmacological agents to investigate specific synaptic receptors and ion channels of auditory neurons, and fluorescent dyes to examine the morphology and location of the neuron being recorded. I am requesting the grant money to purchase essential equipment, including a microscope, a micromanipulator and an osmometer, for a visualized patch clamp recording system. With this system we will study neuronal mechanisms of information processing, plasticity, development, and dysfunction of auditory neurons at cellular and molecular levels. Development of a visualized patch clamp recording system in my laboratory would be greatly valuable to the auditory field, providing a novel direction for future research and ensuring that our laboratory remains on the cutting edge of auditory neuroscience. The advances in auditory neurophysiology that will be made possible by this equipment grant will be of great benefit to Canadian neuroscience. The grant request is urgent as several graduate students in my group require a visualized patch clamp recording system to ensure high quality recordings and reliable results for the proposed research.
Programme
Research Tools and Instruments - Category 1 (<$150,000)
Bénéficiaire
Emplacement
Ontario, Canada
Type de bénéficiaire
Academia
Type
Grant
Source
NSERC's Awards Data
Tel que publié
Application id
345607-2007
Name nom
Wu, ShuHui
Department département
Psychology
Organization id
23
Institution établissement
Carleton University
Province en
Ontario
Province fr
Ontario
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2006
Competition year année de concours
2007
Award amount
61752
Program id
EQPEQ
Program name en
Research Tools and Instruments - Category 1 (<$150,000)
Program name fr
Outils et instruments de recherche - catégorie 1 (<150 000$)
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
1011
Committee name en
Integrative Animal Biology
Committee name fr
Biologie Animale Intégrative
Area of application code
1211
Area of application group en
Advancement of knowledge
Area of application group fr
Avancement des connaissances
Area of application en
Medical and health sciences
Area of application fr
Sciences médicales et de la santé
Research subject code
6303
Research subject group en
Psychology
Research subject group fr
Psychologie
Research subject en
Sensory systems -- auditory
Research subject fr
Systèmes sensoriels -- ouïe
Partie
1
Nb partie
1
Application title
A visualized patch clamp recording system
Keyword
NA
Application summary
A visualized patch clamp recording system is needed to extend my current program of research on the central auditory system (NSERC Discovery Grant supported). The equipment will allow us to carry out the investigation of cellular physiology of the auditory midbrain. The visualized patch clamp recording method has the advantage over blind recordings of allowing detailed investigation of ion channels and improved resolution of physiological events underlying signal processing. The visualized patch clamp recording method is especially necessary for studying developing neurons, such as those in the auditory brainstem of young animals. These neurons are too small or fragile to impale using a sharp electrode recording or a blind patch clamp method. Stable recordings will be achieved with a visualized patch clamp recording system. It is then possible to apply various pharmacological agents to investigate specific synaptic receptors and ion channels of auditory neurons, and fluorescent dyes to examine the morphology and location of the neuron being recorded. I am requesting the grant money to purchase essential equipment, including a microscope, a micromanipulator and an osmometer, for a visualized patch clamp recording system. With this system we will study neuronal mechanisms of information processing, plasticity, development, and dysfunction of auditory neurons at cellular and molecular levels. Development of a visualized patch clamp recording system in my laboratory would be greatly valuable to the auditory field, providing a novel direction for future research and ensuring that our laboratory remains on the cutting edge of auditory neuroscience. The advances in auditory neurophysiology that will be made possible by this equipment grant will be of great benefit to Canadian neuroscience. The grant request is urgent as several graduate students in my group require a visualized patch clamp recording system to ensure high quality recordings and reliable results for the proposed research.
