Grant: Operating Grant

Small differences in our DNA may have drastic effects on the expression levels of our gene. Moreover, even small differences can vastly influence the final composition of the encoded protein. This is due to the process called alternative splicing, whereas different "chunks" of each gene can be excluded or included in the final protein product. This proposal aims to characterize the effect of DNA differences on gene expression levels and types of the final gene products. Using genomics, we will identify large number of differences influencing gene expression. Using computational approaches, we will determine sets of rules that govern the effects of detected polymorphisms - with the final goal of building predictive models. Finaly, using cell culture systems, we will study the phenotypic effects of variation in expression of the affected genes. Our approach will help us understand the role of common genetic variation in determining human phenotypic traits, such as susceptibility to genetic diseases.