Grant: Operating Grant
Université Laval
$581,336.00
Disserting the mechanisms of palmitoylation of the HCV core protein and its influence on formation of lipid droplets; a crucial function in the virus life cycle.
EF 2009-10
Résumé
Hepatitis C virus (HCV) is a plus-strand RNA virus that causes acute and chronic liver disease. More than 3% of the worldwide population is infected, making HCV infection one of the most devastating infectious diseases of our time. The main function of the HCV core protein is the encapsidation of the viral RNA. HCV core plays also an important role in the pathogenesis of the virus since this protein is intimately associated with lipids and can potentially be one of the cause of the disorders observed in the HCV infected patients. This proposal is dedicated to the study of HCV core and its interaction with the lipids. We recently found that palmitoylation (addition of a lipid moety by the cell) of core influences its interaction with cellular membranes, viral assembly and infectivity of the virus. Those events are complex and are related to specific interactions with several host proteins of the lipid metabolism that are not currently well understood. Our major objective is to bring some light of those interactions to hopefully, discover new targets for the development of specific therapies.
Programme
Operating Grant
Bénéficiaire
Emplacement
Quebec, Canada
Type de bénéficiaire
Academia
Type
Grant
Source
CIHR Funding Decisions Database
Tel que publié
Id
199122
Name
Leclerc Denis
Region
Québec
Country
Canada
Orgtype
Institution Paid; Research Institution
Instname2
Infection and Immunity
Theme2
Biomedical
Prcname2
Virology & Viral Pathogenesis
Projecttitle
Disserting the mechanisms of palmitoylation of the HCV core protein and its influence on formation of lipid droplets; a crucial function in the virus life cycle.
Programtype2
Operating Grants
Orgnameinp2
Université Laval
Cihramount2
$581,336
Cihrequipment2
$0
Programname2
Operating Grant
Keyworddelim
5 Cells; Core Protein; Dhhc Enzymes; Endoplasmic Reticulum; Hepatitis C Virus; Huh7; Jfh1; Lipid Droplets; Palmitoylation; Particle Formation; Ubiquitinylation
Abstract
Hepatitis C virus (HCV) is a plus-strand RNA virus that causes acute and chronic liver disease. More than 3% of the worldwide population is infected, making HCV infection one of the most devastating infectious diseases of our time. The main function of the HCV core protein is the encapsidation of the viral RNA. HCV core plays also an important role in the pathogenesis of the virus since this protein is intimately associated with lipids and can potentially be one of the cause of the disorders observed in the HCV infected patients. This proposal is dedicated to the study of HCV core and its interaction with the lipids. We recently found that palmitoylation (addition of a lipid moety by the cell) of core influences its interaction with cellular membranes, viral assembly and infectivity of the virus. Those events are complex and are related to specific interactions with several host proteins of the lipid metabolism that are not currently well understood. Our major objective is to bring some light of those interactions to hopefully, discover new targets for the development of specific therapies.
Competitiondate
201003
Approvedterm2
5 yrs 0 mth
Pinamesdelim
Leclerc, Denis
Approvedterm
5 ans 0 mois
Cihrcontribution2
~ $581,336~ $0~
