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Grant: Alliance Grants

University of Manitoba

$211,957.00

Development of rapid screening methods for measurement of protein quality

EF 2023-24

Résumé
The protein quality of leguminous crops, which depends on amino acid content and digestibility, is a crucial parameter for domestic and global consumers. This research project aims to bridge the gap between breeding programs and end-use quality for peas, the most economically important leguminous crop in Canada, by developing rapid screening methods that can enhance protein traits based on nutritional potential and modernize data for protein labelling claims. This innovative project brings microfluidics, a cutting-edge technology that is revolutionizing biomedicine, into agriculture to breed crops with desired genetic traits. Herein, we aspire to develop a state-of-the-art microfluidics-based lab-on-a-chip and NIR/FTIR/Raman spectroscopy for rapid detection of pulse protein and amino acid content. Our diverse team, which brings together a multidisciplinary team of researchers from the University of Manitoba, the National Research Council, and Canadian Light Source along with representatives from the Canadian pulse sector i.e., Saskatchewan Pulse Growers and Pulse Canada, will execute the project and train a total of twelve highly qualified personnel. The outcome of this seminal project will help plant breeders prioritize end-use quality traits, including techno-functional attributes in varietal development and selection to breed new lines of legumes containing sulphur-rich amino acids. These new varieties will make pulses viable replacements for animal protein products. Moreover, with higher protein quality and utilizing measures like protein digestibility-corrected amino acid score, consumers will be assured that their pulse-based foods contain the proteins stated on food labels.
Programme
Alliance Grants
Emplacement
Manitoba, Canada
Type de bénéficiaire
Academia
Type
Grant
Source
NSERC's Awards Data

Tel que publié

Application id
586312-2023
Name nom
Paliwal, Jitendra J
Department département
Biosystems Engineering
Organization id
19
Institution établissement
University of Manitoba
Province en
Manitoba
Province fr
Manitoba
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2023
Competition year année de concours
2023
Award amount
211957
Program id
ALLRP
Program name en
Alliance Grants
Program name fr
Subventions Alliance
Group en
RESEARCH PARTNERSHIPS
Group fr
PARTENARIATS DE RECHERCHE
Committee code
96
Committee name en
RPP Internal Decision Cttee
Committee name fr
Cté de décision interne - PPR
Area of application code
100
Area of application group en
Agriculture and primary food production
Area of application group fr
Agriculture et produits alimentaires primaires
Area of application en
Agriculture and primary food production
Area of application fr
Agriculture et produits alimentaires primaires
Research subject code
5305
Research subject group en
Molecular biology
Research subject group fr
Protein manipulation and expression
Research subject en
Biologie moléculaire
Research subject fr
Expression et recombinaison protéiques
Application title
Development of rapid screening methods for measurement of protein quality
Keyword
amino acids; breeding; FTIR; lab-on-a-chip; microfluidics; NIR; protein; Raman; SERS; sulphur
Application summary
The protein quality of leguminous crops, which depends on amino acid content and digestibility, is a crucial parameter for domestic and global consumers. This research project aims to bridge the gap between breeding programs and end-use quality for peas, the most economically important leguminous crop in Canada, by developing rapid screening methods that can enhance protein traits based on nutritional potential and modernize data for protein labelling claims. This innovative project brings microfluidics, a cutting-edge technology that is revolutionizing biomedicine, into agriculture to breed crops with desired genetic traits. Herein, we aspire to develop a state-of-the-art microfluidics-based lab-on-a-chip and NIR/FTIR/Raman spectroscopy for rapid detection of pulse protein and amino acid content. Our diverse team, which brings together a multidisciplinary team of researchers from the University of Manitoba, the National Research Council, and Canadian Light Source along with representatives from the Canadian pulse sector i.e., Saskatchewan Pulse Growers and Pulse Canada, will execute the project and train a total of twelve highly qualified personnel. The outcome of this seminal project will help plant breeders prioritize end-use quality traits, including techno-functional attributes in varietal development and selection to breed new lines of legumes containing sulphur-rich amino acids. These new varieties will make pulses viable replacements for animal protein products. Moreover, with higher protein quality and utilizing measures like protein digestibility-corrected amino acid score, consumers will be assured that their pulse-based foods contain the proteins stated on food labels.