Grant: Research Tools and Instruments - Category 1 (<$150,000)

Next-generation DNA sequencing (also called high-throughput sequencing) technologies are revolutionizing the way that scientists do research. These technologies allow the sequencing of millions of base pairs of DNA at one time and provide researchers with the ability to analyze individual genome sequences, complex microbial communities or the expression levels of all genes in one tissue or cell type. Next-generation sequencing is the future of molecular biology research and the availability of a high-throughput sequencer at Memorial University of Newfoundland is essential to maintain a high level of research excellence. In addition, reliable, affordable and guaranteed access to this technology is crucial for researchers to remain competitive with researchers from around the world. The instrument requested will support the research programs of 11 NSERC-funded research labs from 3 different units at Memorial University, spanning a wide spectrum of life science research. These include research in microbiology, marine biology, animal physiology, plant diseases, population genetics, developmental biology and immunology. Analysis of DNA sequences of thousands of different genes simultaneously will allow the applicants to identify sequence changes that contribute to the health, growth, development and immune function of fish, plants and other model and non-model organisms. In addition, research on virus-mediated genetic exchange, wild virus movement and identification of new anti-microbial molecules is also dependent on this technology to advance the knowledge in these research areas.