Grant: Research Tools and Instruments

Advances in microscopy, live cell imaging and image processing have provided profound insight into fundamental cellular processes and behaviors. Obtaining high-resolution images of dynamic processes in live cells requires specialized imaging systems with accompanying environmental controls. The Leica Thunder Imager 3D Assay system is an ideal solution to meet these demands. This inverted widefield microscope features a fully motorized stage, LED8 light source, and an Okolab environmental control chamber. This versatile system also meets our needs for advanced imaging of fixed samples. Unique to the Thunder Imager system is the Instant Computational Clearing (ICC) function, a proprietary system unique to Leica. The ICC technique is the only strategy that will remove the blur surrounding fluorescing objects immediately upon image acquisition. This drastically reduces image processing time, enhances productivity, and allows imaging of thick samples without a confocal microscope. The Thunder Imager will replace an aging Zeiss AxioImagerZ1 microscope. The AxioImagerZ1 played a crucial role in establishing the Copeland lab's research program, however, after 19 years of service it has reached its end of life. In addition, the upright configuration of the Z1 precludes its use for live-cell imaging. The new Thunder Imager will be instrumental in continuing the Copeland lab's research program on cytoskeletal dynamics as well as supporting co-applicant Côté's studies on endosomal phosphoinositides. Additionally, the system will be integrated into the uOttawa Cell Biology and Image Acquisition (CBIA) core facility, providing access to this instrument to more than 400 trainees across 80 research teams at uOttawa and its affiliated Hospitals and Research Institutes. Benefits to HQP training and Canada's leading role in the Natural Sciences: The acquisition of the Thunder Imager 3D Assay system will greatly enhance the live-cell and fixed sample imaging capabilities available to the Ottawa research community, as well as provide new approaches for imaging thick samples such as 3D organoid cultures. These capabilities are vital for the continuity and success of the applicants' research programs and will enable critical discoveries in cell biology and the natural sciences. Integration of this system into the CBIA core will ensure equitable access to cutting-edge imaging technology for all scientists in the uOttawa community. CBIA provides advanced support for the imaging of subcellular dynamics, cellular signaling pathways, cell differentiation, cell-cell interactions, and 3D cell cultures. CBIA offers individualized training in advanced techniques tailored to meet the needs of over 400 highly qualified personnel (HQPs) within our unique and diverse research community. Advanced imaging skills are in high demand for careers in academia, industry, and public health and this training supports Canada's continued leadership in research and technology.