Grant: Research Tools and Instruments - Category 1 (<$150,000)

My research program involves the study of regeneration of cells of the central nervous system, as well as cellular mechanisms underlying learning and memory. The animal model I use in these studies is the mollusc, Lymnaea stagnalis, as its brain cells are large, easily accessible and many are individually identified with known transmitters (e.g. dopamine). Many signaling pathways and neuronal mechanisms are conserved between vertebrates and invertebrates, so research findings from this model may directly influence our knowledge of higher nervous systems. The research I am conducting includes recordings from brain cells in a cultured environment, as well as from intact animals and isolated nervous systems. I currently have three recording set-ups in my laboratory, but only one of these is configured for recordings from cultured cells. My research program has been steadily progressing in the area of cultured neurons, and I now need to convert one of my recording set-ups for use with cultured cells. Most of the equipment required is already in place, but additional equipment required includes an inverted microscope (required for viewing and recording from cultured cells), two hydraulic micromanipulators that will be used for electrophysiological recordings and microinjection techniques, a commercial vibration isolation table that is necessary to allow stable and long-term recordings from cells without causing any damage as well as a pipette puller for voltage-clamp recordings. Without this equipment, my one culture facility will continue to be overutilized by at least seven individuals, which will hinder productivity of my students and my research program.