Grant: Discovery Grants Program - Individual
Brock University
$30,350.00
Chemoenzymatic synthesis and in vitro characterization of glycosylated siRNA
EF 2008-09
Résumé
RNA interference (RNAi) is a recently discovered phenomenon. Small interference RNAs (siRNAs) were found to have the ability to induce degradation of specific messenger RNAs, and hence silence expression of specific genes. Therefore, they are being pursued by pharmaceutical companies as potential therapeutic agents for a wide range of diseases. These development efforts are faced with two major hurdles: the poor stability of siRNAs in serum and the poor efficiency for siRNAs to cross cell membranes. It is proposed here that by linking an appropriately designed carbohydrate molecule to an siRNA molecule, the stability of the siRNA molecule will be improved. More importantly, this modification will provide a handle to enhance the efficiency in transporting siRNA molecules across cell membranes. These combined benefits will facilitate the development of novel RNAi therapeutics to bring relief to patients suffering from certain diseases.
Programme
Discovery Grants Program - Individual
Bénéficiaire
Emplacement
Ontario, Canada
Type de bénéficiaire
Academia
Type
Grant
Source
NSERC's Awards Data
Tel que publié
Application id
326920-2007
Name nom
Yan, Hongbin
Department département
Chemistry
Organization id
22
Institution établissement
Brock University
Province en
Ontario
Province fr
Ontario
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2008
Competition year année de concours
2007
Award amount
30350
Program id
RGPIN
Program nane en
Discovery Grants Program - Individual
Program name fr
Programme de subventions à la découverte - individuelles
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
24
Committee name en
Inorganic & Organic Chemistry
Committee name fr
Chimie inorg. et org.
Area of application code
1202
Area of application group en
Advancement of knowledge
Area of application group fr
Avancement des connaissances
Area of application en
Physical sciences
Area of application fr
Sciences physiques
Research subject code
3705
Research subject group en
Organic chemistry
Research subject group fr
Chimie organique
Research subject en
Bio-organic chemistry
Research subject fr
Chimie bio-organique
Partie
2
Nb partie
3
Application title
Chemoenzymatic synthesis and in vitro characterization of glycosylated siRNA
Keyword
NA
Application summary
RNA interference (RNAi) is a recently discovered phenomenon. Small interference RNAs (siRNAs) were found to have the ability to induce degradation of specific messenger RNAs, and hence silence expression of specific genes. Therefore, they are being pursued by pharmaceutical companies as potential therapeutic agents for a wide range of diseases. These development efforts are faced with two major hurdles: the poor stability of siRNAs in serum and the poor efficiency for siRNAs to cross cell membranes. It is proposed here that by linking an appropriately designed carbohydrate molecule to an siRNA molecule, the stability of the siRNA molecule will be improved. More importantly, this modification will provide a handle to enhance the efficiency in transporting siRNA molecules across cell membranes. These combined benefits will facilitate the development of novel RNAi therapeutics to bring relief to patients suffering from certain diseases.
