Grant: Research Tools and Instruments - Category 1 (<$150,000)
University of Waterloo
$136,544.00
Stopped/quenched flow system
EF 2007-08
Résumé
We are requesting funding to obtain a stopped/quenched flow system for measuring rapid reaction kinetics (on a timescale of millisecond to minute) and steady-state properties of a wide range of molecules (proteins, peptides, drugs, ribozymes). The requested equipment consists of 2 components: 1) a rapid mixing apparatus, for initiating various reactions of these molecules by mixing up to 4 different solutions, in 3 mixing steps, with variable delay times between mixing, and 2) a spectrometer capable of measuring fluorescence, light scattering and absorbance, to monitor the progress of the reactions. The co-applicants (6 biochemists and chemists, and 1 chemical engineer, whose groups currently include a total of 81 researchers) need to use this equipment on a routine, daily basis for making extensive measurements that form a central part of their research programs on: protein folding, aggregation, and engineering; enzyme and ribozyme catalyst function and inhibition; and bio-nanomaterials. This research is important for understanding natural and disease processes in biology, and from this developing new therapeutics. The co-applicants currently have just one aging stopped/quenched flow system: this instrumentation has increasingly limited capabilities due to the progressive failure of components, which often cannot be replaced, and it is becoming very unreliable in its operation. The state of this instrumentation is very negatively impacting the research efforts of the co-applicants, due to failed experiments that need to be repeated using costly reagents, time required to trouble shoot and attempt instrument repairs, and instrument down-time. Thus, the co-applicants urgently require the requested equipment to pursue many key elements of their research programs. The requested equipment will provide very valuable training for many highly qualified personnel, including undergraduate students, technicians, graduate students, and postdoctoral fellows, in using this powerful and widely applicable instrumentation.
Programme
Research Tools and Instruments - Category 1 (<$150,000)
Bénéficiaire
Emplacement
Ontario, Canada
Type de bénéficiaire
Academia
Type
Grant
Source
NSERC's Awards Data
Tel que publié
Application id
360097-2008
Name nom
Meiering, Elizabeth
Department département
Chemistry
Organization id
33
Institution établissement
University of Waterloo
Province en
Ontario
Province fr
Ontario
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2007
Competition year année de concours
2008
Award amount
136544
Program id
EQPEQ
Program name en
Research Tools and Instruments - Category 1 (<$150,000)
Program name fr
Outils et instruments de recherche - catégorie 1 (<150 000$)
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
24
Committee name en
Inorganic & Organic Chemistry
Committee name fr
Chimie inorg. et org.
Area of application code
1201
Area of application group en
Advancement of knowledge
Area of application group fr
Avancement des connaissances
Area of application en
Life sciences (including biotechnology)
Area of application fr
Sciences de la vie (y compris biotechnologie)
Research subject code
5403
Research subject group en
Biochemistry
Research subject group fr
Biochimie
Research subject en
Proteins and peptides, amino acids
Research subject fr
Protéines et peptides, acides aminés
Partie
1
Nb partie
1
Application title
Stopped/quenched flow system
Keyword
NA
Application summary
We are requesting funding to obtain a stopped/quenched flow system for measuring rapid reaction kinetics (on a timescale of millisecond to minute) and steady-state properties of a wide range of molecules (proteins, peptides, drugs, ribozymes). The requested equipment consists of 2 components: 1) a rapid mixing apparatus, for initiating various reactions of these molecules by mixing up to 4 different solutions, in 3 mixing steps, with variable delay times between mixing, and 2) a spectrometer capable of measuring fluorescence, light scattering and absorbance, to monitor the progress of the reactions. The co-applicants (6 biochemists and chemists, and 1 chemical engineer, whose groups currently include a total of 81 researchers) need to use this equipment on a routine, daily basis for making extensive measurements that form a central part of their research programs on: protein folding, aggregation, and engineering; enzyme and ribozyme catalyst function and inhibition; and bio-nanomaterials. This research is important for understanding natural and disease processes in biology, and from this developing new therapeutics. The co-applicants currently have just one aging stopped/quenched flow system: this instrumentation has increasingly limited capabilities due to the progressive failure of components, which often cannot be replaced, and it is becoming very unreliable in its operation. The state of this instrumentation is very negatively impacting the research efforts of the co-applicants, due to failed experiments that need to be repeated using costly reagents, time required to trouble shoot and attempt instrument repairs, and instrument down-time. Thus, the co-applicants urgently require the requested equipment to pursue many key elements of their research programs. The requested equipment will provide very valuable training for many highly qualified personnel, including undergraduate students, technicians, graduate students, and postdoctoral fellows, in using this powerful and widely applicable instrumentation.
