Grant: Canada Research Chairs

The main goal of the proposal is to better understand and to reconstruct the Amaryllidaceae alkaloid biosynthetic pathways in microalgae. The program involves three key objectives: Obj. 1. Identification of Novel Alkaloid Biosynthesis Genes: Utilizing integrated multi-omics and artificial intelligence (AI) approaches, we aim to identify novel genes involved in alkaloid metabolism. This entails mining extensive transcriptome and metabolome databases, employing machine learning techniques, and developing predictive models to elucidate complex metabolic pathways. Obj. 2. Characterization of Candidate Genes: Thorough characterization of candidate genes implicated in alkaloid biosynthesis will be conducted using a multidisciplinary approach. This involves bioinformatics analysis, gene expression profiling, protein localization studies, biochemical assays, and functional genomics to validate gene function and lay the groundwork for metabolic engineering. Obj. 3. Engineering Microalgae for Alkaloid Production: We propose to engineer microalgae as sustainable biofactories for alkaloid production, leveraging their unique advantages such as rapid growth, genetic flexibility, and photosynthetic capabilities. By reconstructing alkaloid biosynthetic pathways in microalgae, we aim to produce bioactive alkaloids at scale while minimizing environmental impact. Our research program integrates cutting-edge technologies and interdisciplinary methodologies to address key challenges in alkaloid biosynthesis and production. We combine systems biology, bioinformatics, biochemical analysis, molecular genetics, and metabolic engineering to advance our understanding of plant specialized metabolism and develop innovative strategies for alkaloid production. This research program offers a groundbreaking approach to phytomedicine and environmental conservation by harnessing the potential of microalgae for sustainable alkaloid production. By identifying novel alkaloid biosynthetic genes and characterizing their functions, we pave the way for metabolic engineering efforts aimed at enhancing alkaloid production in microalgae. This innovative approach not only addresses the growing demand for bioactive alkaloids but also contributes to environmental sustainability through CO2 fixation. Ultimately, our research program aims to train the next generation of scientists and provide novel solutions to global health challenges while promoting clean and renewable energy technologies.