Grant: Operating Grant - PA: Genetics (Bridge Funding: Research Priorities)

Oligodendroglia is a specialized cell type that covers the axons of neurons in the central nervous system. Studying how these cells function would help us advance research into diseases related to these types of cells, such as multiple sclerosis (MS). In this project, we will generate DNA sequences using computers (artificial intelligence) to turn on genes in oligodendroglia. First, we will generate data from oligodendroglia cells grown in the lab as well as cells from mice. Next, we will use these data to teach computers to design sequences that will only work in oligodendroglia. Then, we will test these sequences in two types of cells (oligodendroglia and an unrelated cell type). Finally, using a type of gene therapy in mice, we will test sequences that worked in oligodendroglia cells but not the other cells to see if they behave the same in the brain. If successful, the resulting research will be useful for future gene therapy. The designed DNA sequences could be used to turn on genes that would fix oligodendroglia in neurological diseases like MS.