Grant: Research Tools and Instruments - Category 1 (<$150,000)
University of Alberta
$145,297.00
Microarray scanner for functional genomics research
EF 2007-08
Résumé
The functional genomics (transcriptomics) and proteomics is the major focus of research conducted in a multi-user, multi-faculty Agricultural Genomics and Proteomics Unit (AGP Unit) in the Department of Agricultural, Food and Nutritional Science Various groups conducting this research include The Swine Reproduction-Development Program, The Bovine Genomics Initiative, Plant Biotechnology Group, Canola Biotechnology Program and Plant Pathology Group supporting the research programs of 60 students, technicians, post doctoral fellows and research associates. The acquisition of a "High-resolution, multiple laser Microarray Scanner with analytical software" is very critical for functional genomics research. The mRNA transcriptome serves as the source of genetic blueprints that are utilized as the code for cellular proteins that can function as structural building blocks, enzymes and nutrient transporters and other essential cell components. The measurement of gene expression, through transcriptome analysis allows for determination of levels of cellular genes that vary with respect to specific timelines or treatments. The requested microarray scanner is an essential piece of equipment that allows simultaneous comparison of 10,000-50,000 genes present on a hybridized microarray chip. This requested scanner retains the necessary high levels of sensitivity, accuracy and reproducibility for effective comparison of transcriptomes from differentially dye-labeled cDNA. Furthermore, the feature extraction software available with the scanner can read and process up to 100 raw microarray image files in an automated mode. The incorporation of sensitive and reliable transcriptome analysis into our research programs will translate into a corresponding exponential increase in the capacity, and efficacy by which we can provide insights into the complex eukaryotic physiological processes that we are striving to understand. The requested microarray scanner will be utilized within the current research programs of the 8 applicants and will also allow additional colleagues to incorporate transcriptome analysis using high-density microarrays into future research endeavours.
Programme
Research Tools and Instruments - Category 1 (<$150,000)
Bénéficiaire
Emplacement
Alberta, Canada
Type de bénéficiaire
Academia
Type
Grant
Source
NSERC's Awards Data
Tel que publié
Application id
360066-2008
Name nom
Dixon, Walter
Department département
Agricultural, Food & Nutritional Sc
Organization id
9
Institution établissement
University of Alberta
Province en
Alberta
Province fr
Alberta
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2007
Competition year année de concours
2008
Award amount
145297
Program id
EQPEQ
Program name en
Research Tools and Instruments - Category 1 (<$150,000)
Program name fr
Outils et instruments de recherche - catégorie 1 (<150 000$)
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
32
Committee name en
Cell Biology
Committee name fr
Biologie cellulaire
Area of application code
1209
Area of application group en
Advancement of knowledge
Area of application group fr
Avancement des connaissances
Area of application en
Agriculture
Area of application fr
Agriculture
Research subject code
5302
Research subject group en
Molecular biology
Research subject group fr
Biologie moléculaire
Research subject en
Gene expression
Research subject fr
Expression génétique
Partie
1
Nb partie
1
Application title
Microarray scanner for functional genomics research
Keyword
NA
Application summary
The functional genomics (transcriptomics) and proteomics is the major focus of research conducted in a multi-user, multi-faculty Agricultural Genomics and Proteomics Unit (AGP Unit) in the Department of Agricultural, Food and Nutritional Science Various groups conducting this research include The Swine Reproduction-Development Program, The Bovine Genomics Initiative, Plant Biotechnology Group, Canola Biotechnology Program and Plant Pathology Group supporting the research programs of 60 students, technicians, post doctoral fellows and research associates. The acquisition of a "High-resolution, multiple laser Microarray Scanner with analytical software" is very critical for functional genomics research. The mRNA transcriptome serves as the source of genetic blueprints that are utilized as the code for cellular proteins that can function as structural building blocks, enzymes and nutrient transporters and other essential cell components. The measurement of gene expression, through transcriptome analysis allows for determination of levels of cellular genes that vary with respect to specific timelines or treatments. The requested microarray scanner is an essential piece of equipment that allows simultaneous comparison of 10,000-50,000 genes present on a hybridized microarray chip. This requested scanner retains the necessary high levels of sensitivity, accuracy and reproducibility for effective comparison of transcriptomes from differentially dye-labeled cDNA. Furthermore, the feature extraction software available with the scanner can read and process up to 100 raw microarray image files in an automated mode. The incorporation of sensitive and reliable transcriptome analysis into our research programs will translate into a corresponding exponential increase in the capacity, and efficacy by which we can provide insights into the complex eukaryotic physiological processes that we are striving to understand. The requested microarray scanner will be utilized within the current research programs of the 8 applicants and will also allow additional colleagues to incorporate transcriptome analysis using high-density microarrays into future research endeavours.
