Grant: Discovery Grants Program - Individual

Secondary electrons are the most abundant species produced by high-energy radiation in matter; most have energies below 20 eV. Such low-energy electrons (LEEs) play key roles in a range of fields, including astrochemistry, photochemistry and radiation therapy. To understand and/or control of such phenomena, data is needed on LEE-induced processes occuring within, and at the interfaces of, molecular and biomolecular materials. Our objective during the next five years is to study LEE related phenomena in two distinct scientifically significant areas: i) LEE induced damage to DNA and DNA complexes (DNAC, formed by binding potential or clinically-available radiosensitizers or chemotherapeutic agents to DNA): We will characterize the fundamental mechanisms of LEE interactions in DNA and DNAC in targets of increasing complexity (e.g., films of DNA subunits, oligonucleotides, plasmid DNA, etc.,) under experimental conditions that progressively approach those encountered in cells. This will be done by coadsorbing in these films O2, H2O, amino acids and mixtures thereof, under ultrahigh vacuum (UHV) or at atmospheric pressure. Thus, this research seeks an improved understanding of LEE-induced processes in DNA and DNAC in close contact with cellular molecules. To achieve this goal, we will continue to develop state-of-the-art bimolecular thin-film technology and new LEE sources. Since DNA is the target in radiotherapy, this work will help identify new modalities and molecular agents to improve cancer treatment with radiotherapy alone or combined with chemotherapy. Previous research on these fundamental aspects of LEE-induced radiation damage to biomolecules was supported by the CIHR, but presently CIHR funding is more appropriately requested only for the clinical applications of such work. ii) Astrochemistry: radiation processing of molecular ices, like to those found in space environments is of considerable current interest for the interpretation of astronomical data and the synthesis of complex molecules. The chemistry initiated by LEEs in such ices may thus relates to the formation of pre-biotic molecules by cosmic and UV irradiation and the origins of life. We will continue our studies into LEE-driven chemistry on thin-film ices formed in UHV. These will contain mixtures of molecules such as H2O, NH3, CO2, CO and CH4 that condense upon dust grains in the interstellar medium and are present in comets and planetary ices. Building on our recent work on the identification of glycine in LEE-irradiated ices, we will attempt to investigate the formation of other amino acids, small peptides, DNA bases and related molecules. In both areas, we will focus on the identification of basic physical mechanisms that dominate product yields, including transient anion formation. This research is intended to promote the highest levels of research quality in the field of LEE-molecule interactions, spur academic interest and provide a stimulating training environment.