Grant: Operating Grant
University of Toronto
$763,865.00
Roles of human RNA polymerase II CTD-interacting proteins in the regulation of gene expression
EF 2011-12
Résumé
The regulated expression of human genes determines the developmental fates of human cells, and aberrations in this process often lead to cancer. RNA polymerase II, the enzyme that transcribes most human genes, must initiate transcription in the right places and at the correct frequencies, elongate efficiently through genes, and terminate transcription in the right places, and the RNA molecules produced by RNA polymerase II must be correctly modified. We have identified three human proteins, RPRD1A, RPRD1B, and RPRD2, about which virtually nothing is known and which interact with particular forms of RNA polymerase II. We now propose to investigate how these proteins, which are produced in all kinds of human tissues, regulate the various steps in the expression of human genes and, perhaps, how they are related to human disease.
Programme
Operating Grant
Bénéficiaire
Emplacement
Ontario, Canada
Type de bénéficiaire
Academia
Type
Grant
Source
CIHR Funding Decisions Database
Tel que publié
Id
238230
Name
Greenblatt Jack F
Region
Ontario
Country
Canada
Orgtype
Institution Paid; Research Institution
Instname2
Genetics
Theme2
Biomedical
Prcname2
Biochemistry & Molecular Biology - B
Projecttitle
Roles of human RNA polymerase II CTD-interacting proteins in the regulation of gene expression
Programtype2
Operating Grants
Orgnameinp2
University of Toronto
Cihramount2
$763,865
Cihrequipment2
$0
Programname2
Operating Grant
Keyworddelim
Chip-Sequencing; Chromatin Immunoprecipitation; Ctd; Human Tissue Culture Cells; Mass Spectrometry; Phosphorylation; Rna Polymerase Ii; Rprd Proteins; Transcription
Abstract
The regulated expression of human genes determines the developmental fates of human cells, and aberrations in this process often lead to cancer. RNA polymerase II, the enzyme that transcribes most human genes, must initiate transcription in the right places and at the correct frequencies, elongate efficiently through genes, and terminate transcription in the right places, and the RNA molecules produced by RNA polymerase II must be correctly modified. We have identified three human proteins, RPRD1A, RPRD1B, and RPRD2, about which virtually nothing is known and which interact with particular forms of RNA polymerase II. We now propose to investigate how these proteins, which are produced in all kinds of human tissues, regulate the various steps in the expression of human genes and, perhaps, how they are related to human disease.
Competitiondate
201109
Approvedterm2
5 yrs 0 mth
Pinamesdelim
Greenblatt, Jack F
Approvedterm
5 ans 0 mois
Cihrcontribution2
~ $763,865~ $0~
