Grant: Research Tools and Instruments - Category 1 (<$150,000)
University of Western Ontario
$149,463.00
LC-MS for the Analysis of Small Molecules
EF 2011-12
Résumé
We are seeking funds to acquire the mass spectrometer component of an LC-MS system to be used for small molecule analysis. Specifically, we are asking for funds to purchase an Agilent Single Quad ES detector. We will acquire funds through an alternative source for the companion 1260 Infinity UHPLC system. The availability of in-house LC-MS facilities will support the research programs of seven applicants. Four of these (Bernards, Huner and the Grbics) will use the system for plant hormone analysis. These molecules are present in very low abundance, yet have significant impact on overall metabolism (especially in response to stress). LC-MS is the most sensitive and appropriate tool for plant hormone anlysis. Other applicants will use the system for the analysis of root exudates involved in plant response to metal stress (Macfie), bacterial hopanoids (Valvano) and siderophores (Heinrichs). The data collected will address fundamental questions of plant and bacterial stress biology.
Other LC-MS facilities available at Western are set up for protein analysis; routine small molecule analysis by LC-MS is not otherwise available.
Programme
Research Tools and Instruments - Category 1 (<$150,000)
Bénéficiaire
Emplacement
Ontario, Canada
Type de bénéficiaire
Academia
Type
Grant
Source
NSERC's Awards Data
Tel que publié
Application id
422571-2012
Name nom
Bernards, Mark
Department département
Biology
Organization id
36
Institution établissement
University of Western Ontario
Province en
Ontario
Province fr
Ontario
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2011
Competition year année de concours
2012
Award amount
149463
Program id
EQPEQ
Program nane en
Research Tools and Instruments - Category 1 (<$150,000)
Program name fr
Outils et instruments de recherche - catégorie 1 (<150 000$)
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
1502
Committee name en
Biological Systems and Functions
Committee name fr
Systèmes et fonctions biologiques
Area of application code
1200
Area of application group en
Advancement of knowledge
Area of application group fr
Avancement des connaissances
Area of application en
Advancement of knowledge
Area of application fr
Avancement des connaissances
Research subject code
4900
Research subject group en
Plant and treebiology
Research subject group fr
Biologie végétale et des arbres
Research subject en
Plant and treebiology
Research subject fr
Biologie végétale et des arbres
Partie
1
Nb partie
1
Application title
LC-MS for the Analysis of Small Molecules
Keyword
NSERC
Application summary
We are seeking funds to acquire the mass spectrometer component of an LC-MS system to be used for small molecule analysis. Specifically, we are asking for funds to purchase an Agilent Single Quad ES detector. We will acquire funds through an alternative source for the companion 1260 Infinity UHPLC system. The availability of in-house LC-MS facilities will support the research programs of seven applicants. Four of these (Bernards, Huner and the Grbics) will use the system for plant hormone analysis. These molecules are present in very low abundance, yet have significant impact on overall metabolism (especially in response to stress). LC-MS is the most sensitive and appropriate tool for plant hormone anlysis. Other applicants will use the system for the analysis of root exudates involved in plant response to metal stress (Macfie), bacterial hopanoids (Valvano) and siderophores (Heinrichs). The data collected will address fundamental questions of plant and bacterial stress biology.
Other LC-MS facilities available at Western are set up for protein analysis; routine small molecule analysis by LC-MS is not otherwise available.
