Grant: Research Tools and Instruments

Our group has long been at the forefront of research on new bioorthogonal probes and characterizing host-virus interactions. However, with the recent breakdown of our former multi-mode plate reader, a SpectraMax i3 which is no longer in service, we are now unable to conduct many essential assays and analyses for characterizing new probes and studying molecular interactions. This has resulted in a significant disruption in our research workflow, and reduced training opportunities for both new and experienced HQPs. Here, we request funding to purchase a new multi-mode plate reader, the SpectraMax i3x, replacing the broken plate reader we owned previously. Unlike many other basic plate readers currently on the market, this instrument's functionality in TR-FRET, Fluorescence Polarization, and many other kinetic assays made this an ideal instrument for the characterization of many bioorthogonal probes we have developed. The SpectraMax i3x is equipped with highly advanced detection technologies including TR-FRET, fluorescence polarization, and luminescence allowing for highly precise monitoring of interactions between synthesized probes and viral/host cell proteins. This multi-mode plate reader enhances our research capabilities by enabling us to conduct a wide range of assays for understanding complex biological processes. Additionally, this system's "cartridge"-based designs provide flexibility for future assay development, thus remaining aligned with the evolving needs of uOttawa's research community. Our ongoing research has become increasingly reliant on characterizing new probes and ligands for monitoring host-virus interactions. By replacing our broken multi-mode plate reader with the SpectraMax i3x, we will restore the research output of dozens of HQPs who will actively use this instrument and contribute greatly to the advancement of our scientific goals.