Grant: Research Tools and Instruments
Université de Sherbrooke
$149,885.00
High-throughput nucleic acid detection platform
EF 2015-16
Résumé
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<span style=""font-size:12.0pt;font-family:Helvetica;
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mso-ansi-language:EN-US;mso-fareast-language:JA;mso-bidi-language:AR-SA"" lang=""EN-US"">The
capacity to separate and analyze nucleic acid is an essential component of life
sciences and the backbone of molecular biology research. The ability to quickly
and precisely identify RNA and DNA fragments with discreet sizes provides
powerful tools for understanding the mechanism of gene expression and determining
the regulatory elements that affect RNA production and stability. By using
microfluidics capillary electrophoresis we developed methods for
high-throughput analysis of messenger RNA splice variants that are now being
used by many researchers worldwide. Indeed, microfluidics capillary
electrophoresis rapidly became the backbone of the Université de Sherbrooke
RNomics platform that caters to the needs of basic researchers interested in
RNA biology. The goal of this application is to acquire replacements for the
automated nucleic acid separation equipment needed to maintain the service of
the RNomics platform. It is essential for our institute to maintain its
capacity to provide high-throughput microfluidic electrophoresis in order to
prevent the disruption of the NSERC-funded research programs requiring the
detection and separation of nucleic acid fragments.
Programme
Research Tools and Instruments
Bénéficiaire
Emplacement
Quebec, Canada
Type de bénéficiaire
Academia
Type
Grant
Source
NSERC's Awards Data
Tel que publié
Application id
RTI-2016-00045
Name nom
AbouElela, Sherif
Department département
Microbiologie et d'infectiologie - Microbiologie et d'infectiologie
Organization id
68
Institution établissement
Université de Sherbrooke
Province en
Québec
Province fr
Québec
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2015
Competition year année de concours
2016
Award amount
149885
Program id
RTI
Program name en
Research Tools and Instruments
Program name fr
Outils et instruments de recherche
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
1601
Committee name en
Genes, Cells and Molecules (RTI)
Committee name fr
Gènes, cellules et molécules (OIR)
Area of application code
1211
Area of application group en
Advancement of knowledge
Area of application group fr
Avancement des connaissances
Area of application en
Medical and health sciences
Area of application fr
Sciences médicales et de la santé
Research subject code
5302
Research subject group en
Molecular biology
Research subject group fr
Biologie moléculaire
Research subject en
Gene expression
Research subject fr
Expression génétique
Partie
1
Nb partie
1
Application title
High-throughput nucleic acid detection platform
Keyword
NSERC
Application summary
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capacity to separate and analyze nucleic acid is an essential component of life
sciences and the backbone of molecular biology research. The ability to quickly
and precisely identify RNA and DNA fragments with discreet sizes provides
powerful tools for understanding the mechanism of gene expression and determining
the regulatory elements that affect RNA production and stability. By using
microfluidics capillary electrophoresis we developed methods for
high-throughput analysis of messenger RNA splice variants that are now being
used by many researchers worldwide. Indeed, microfluidics capillary
electrophoresis rapidly became the backbone of the Université de Sherbrooke
RNomics platform that caters to the needs of basic researchers interested in
RNA biology. The goal of this application is to acquire replacements for the
automated nucleic acid separation equipment needed to maintain the service of
the RNomics platform. It is essential for our institute to maintain its
capacity to provide high-throughput microfluidic electrophoresis in order to
prevent the disruption of the NSERC-funded research programs requiring the
detection and separation of nucleic acid fragments.</span>
