Grant: Project Grant
University of British Columbia
$573,375.00
The network biology of stem cell derived macrophages; Deciphering the role of cellular reprogramming in sepsis
EF 2015-16
Résumé
Sepsis is a leading cause of death and disability that annually causes 5 million deaths worldwide (8.3% of all deaths). Sepsis is defined as an infection combined with an aberrant inflammatory response; severe sepsis includes organ dysfunction. We have identified a gene expression signature that predicts the onset of sepsis and organ dysfunction, and this signature is associated with bacteria-eating cells termed macrophages that are unable to respond to bacteria. Here we are proposing to clarify the biological roles of the genes in this signature by characterizing macrophages made from skin-derived stem cells that have been mutated in each of the genes involved in the sepsis signature. This will allow us a deeper understanding of the pathology of sepsis.
Programme
Project Grant
Bénéficiaire
Emplacement
British Columbia, Canada
Type de bénéficiaire
Academia
Type
Grant
Source
CIHR Funding Decisions Database
Tel que publié
Id
343310
Name
Hancock Robert Ernest W
Region
British Columbia
Country
Canada
Orgtype
Institution Paid; Research Institution
Instname2
Genetics
Theme2
Biomedical
Prcname2
Project Grant Competition
Projecttitle
The network biology of stem cell derived macrophages; Deciphering the role of cellular reprogramming in sepsis
Programtype2
Operating Grants
Orgnameinp2
University of British Columbia
Cihramount2
$573,375
Cihrequipment2
$0
Programname2
Project Grant
Keyworddelim
Bioinformatics; Cas9-Crispr Technology; Cellular Reprogramming; Induced Pluripotent Stem Cells; Macrophages; Mutants; Network Biology; Rna-Seq; Sepsis
Abstract
Sepsis is a leading cause of death and disability that annually causes 5 million deaths worldwide (8.3% of all deaths). Sepsis is defined as an infection combined with an aberrant inflammatory response; severe sepsis includes organ dysfunction. We have identified a gene expression signature that predicts the onset of sepsis and organ dysfunction, and this signature is associated with bacteria-eating cells termed macrophages that are unable to respond to bacteria. Here we are proposing to clarify the biological roles of the genes in this signature by characterizing macrophages made from skin-derived stem cells that have been mutated in each of the genes involved in the sepsis signature. This will allow us a deeper understanding of the pathology of sepsis.
Competitiondate
201603
Approvedterm2
5 yrs 0 mth
Pinamesdelim
Hancock, Robert Ernest W
Approvedterm
5 ans 0 mois
Cihrcontribution2
~ $573,375~ $0~
