Grant: Research Tools and Instruments - Category 1 (<$150,000)
York University
$136,171.00
Characterization of bio-molecular interactions and investigation of catalytic mechanisms of bio- and chemical catalysts by micro-Isothermal Titration Calorimetry
EF 2011-12
Résumé
In this proposal, we aim to establish an infrastructure for characterization of biomolecular interactions, catalytic mechanisms of bio and chemical catalysts and engennering of ""smart"" molecular surfaces. We are requesting a micro Isothermal Titration Calorimeter (microITC). Isothermal titration calorimetry measures the heat released from biological and chemical reactions. The sensitivity of the requested microITC is such that it will enable characterization of reactions that have high to low heat release and binding affinities from 103 to 1012 M-1. Hence, it enable characterization of a wide range of interactions such as those between DNA-transcription factors, which normally have binding affinities at nanomolar range, as well as protein-protein/peptide/small molecules interactions that normally have binding affinities from low micromolar to high micromolar. At the same time, the high sensitivity of such instrument would enable for quick screening of aptamers and small libraries of small molecules as well evaluation of the efficiency of delivery of modified lipids to cells . Based on these capacities this instrument will greatly enhance the current research capabilities of the applicants of this proposal to study, but not limited, to bacterial response to cell wall stress, antibiotic resistance, formation of protein-based nanotubes and biocells, mechanism of the on-set of Alzheimer's disease, identification and development of drugs and design and development of chemical catalysts and engineering of ""smart"" surfaces for controlling of stem cell differentiation. These research areas are essential to the economy and health of Canadians. As many as 35 high quality personnel (HQPs), coming from six different research laboratories, will be trained in this state-of-the-art instrument. Utilization of this instrument in industrial settings will enable the HQPs to be fit well in this sector. As well, for those that will advance their careers in academia, the gained expertise, will enable them to advance other research areas and train more HQPs.
Programme
Research Tools and Instruments - Category 1 (<$150,000)
Bénéficiaire
Emplacement
Ontario, Canada
Type de bénéficiaire
Academia
Type
Grant
Source
NSERC's Awards Data
Tel que publié
Application id
423008-2012
Name nom
GolemiKotra, Dasantila
Department département
Chemistry
Organization id
38
Institution établissement
York University
Province en
Ontario
Province fr
Ontario
Country en
CANADA
Country fr
CANADA
Fiscal year exercice financier
2011
Competition year année de concours
2012
Award amount
136171
Program id
EQPEQ
Program nane en
Research Tools and Instruments - Category 1 (<$150,000)
Program name fr
Outils et instruments de recherche - catégorie 1 (<150 000$)
Group en
Discovery grants programs
Group fr
Programmes de subventions à la découverte
Committee code
1501
Committee name en
Genes, Cells and Molecules
Committee name fr
Gènes, cellules et molécules
Area of application code
1211
Area of application group en
Advancement of knowledge
Area of application group fr
Avancement des connaissances
Area of application en
Medical and health sciences
Area of application fr
Sciences médicales et de la santé
Research subject code
3504
Research subject group en
Analytical chemistry
Research subject group fr
Chimie analytique
Research subject en
Bioanalytical chemistry
Research subject fr
Chimie bioanalytique
Partie
1
Nb partie
1
Application title
Characterization of bio-molecular interactions and investigation of catalytic mechanisms of bio- and chemical catalysts by micro-Isothermal Titration Calorimetry
Keyword
NSERC
Application summary
In this proposal, we aim to establish an infrastructure for characterization of biomolecular interactions, catalytic mechanisms of bio and chemical catalysts and engennering of ""smart"" molecular surfaces. We are requesting a micro Isothermal Titration Calorimeter (microITC). Isothermal titration calorimetry measures the heat released from biological and chemical reactions. The sensitivity of the requested microITC is such that it will enable characterization of reactions that have high to low heat release and binding affinities from 103 to 1012 M-1. Hence, it enable characterization of a wide range of interactions such as those between DNA-transcription factors, which normally have binding affinities at nanomolar range, as well as protein-protein/peptide/small molecules interactions that normally have binding affinities from low micromolar to high micromolar. At the same time, the high sensitivity of such instrument would enable for quick screening of aptamers and small libraries of small molecules as well evaluation of the efficiency of delivery of modified lipids to cells . Based on these capacities this instrument will greatly enhance the current research capabilities of the applicants of this proposal to study, but not limited, to bacterial response to cell wall stress, antibiotic resistance, formation of protein-based nanotubes and biocells, mechanism of the on-set of Alzheimer's disease, identification and development of drugs and design and development of chemical catalysts and engineering of ""smart"" surfaces for controlling of stem cell differentiation. These research areas are essential to the economy and health of Canadians. As many as 35 high quality personnel (HQPs), coming from six different research laboratories, will be trained in this state-of-the-art instrument. Utilization of this instrument in industrial settings will enable the HQPs to be fit well in this sector. As well, for those that will advance their careers in academia, the gained expertise, will enable them to advance other research areas and train more HQPs.
