Grant: Idea to Innovation

The objective of the proposed research is to improve the innovative molecular multiplex diagnostic assay that we conceptualized and initiated during the last two years into a form or product that can be utilized for detection of pathogens that affect salmon. The innovative product will detect simultaneously the 6 globally detrimental and economically damaging pathogens of salmon in a single sample in around 5 hours which are IHNV, ISAV, IPNV, SAV, VHSV and R. salmoninarum (the causative agent of BKD). Laboratory and field validation will be performed using the guidelines of World Organisation of Animal Health (OIE). The multiplex assay is expected to have higher or similar sensitivity and specificity, faster turnaround time and will be less costly on a per-test basis than the existing molecular diagnostic assay such as traditional PCR and real time PCR. This proposal will improve, characterize and document the performance of the novel assay so it can be marketed and utilized by potential users (salmon producers, regulatory agencies, veterinarians, aquatic specialists, epidemiologists, researchers, vaccine companies, etc.). After validation, the multiplex assay will be offered in a non-kit format at AVC-UPEI and can be licensed to private and government laboratories in Canada. It will be developed into a kit by an industry partner for marketing regionally, nationally and globally.The assay will aid in the diagnosis during viral outbreaks and can be incorporated into surveillance programs.