Grant: Travel Awards - Institute Community Support
Direct Payment
$1,000.00
Investigating the role of the DNA damage response in the EBV lytic cycle
EF 2012-13
Résumé
Several DNA damage response (DDR) proteins such as ATM, Chk2 and H2AX have been shown to be activated during Epstein Barr Virus (EBV) lytic replication. In addition, these DDR proteins have been shown colocalize to sites of lytic EBV DNA replication. Although DDR proteins are known to be at sites of lytic replication, their role in EBV lytic replication is not well established. We have induced EBV lytic replication in the EBV-positive gastric cancer cell line AGS-EBV using sodium butyrate and TpA and followed the activation of ATM and H2AX over 24 hours. In these experiments we have found that ATM and H2AX are activated prior to the EBV processivity factor BMRF1 but not the EBV transcription factor BZLF1. In addition, we have found that silencing of ATM and H2AX alter levels of EBV proteins during lytic replication.
Programme
Travel Awards - Institute Community Support
Bénéficiaire
Emplacement
Ontario, Canada
Type
Grant
Source
CIHR Funding Decisions Database
Tel que publié
Id
262877
Name
Malik Natasha
Region
Ontario
Country
Canada
Orgtype
Research Institution
Instname2
Cancer Research
Theme2
Biomedical
Prcname2
Institute of Cancer Research
Projecttitle
Investigating the role of the DNA damage response in the EBV lytic cycle
Programtype2
Undefined
Orgnameinp2
Direct Payment
Cihramount2
$1,000
Cihrequipment2
$0
Programname2
Travel Awards - Institute Community Support
Keyworddelim
Cancer; Dna Damage; Viral Lytic Reactivation; Virus-Host Interaction
Abstract
Several DNA damage response (DDR) proteins such as ATM, Chk2 and H2AX have been shown to be activated during Epstein Barr Virus (EBV) lytic replication. In addition, these DDR proteins have been shown colocalize to sites of lytic EBV DNA replication. Although DDR proteins are known to be at sites of lytic replication, their role in EBV lytic replication is not well established. We have induced EBV lytic replication in the EBV-positive gastric cancer cell line AGS-EBV using sodium butyrate and TpA and followed the activation of ATM and H2AX over 24 hours. In these experiments we have found that ATM and H2AX are activated prior to the EBV processivity factor BMRF1 but not the EBV transcription factor BZLF1. In addition, we have found that silencing of ATM and H2AX alter levels of EBV proteins during lytic replication.
Competitiondate
201205
Approvedterm2
0 yr 3 mths
Pinamesdelim
Malik, Natasha
Approvedterm
0 an 3 mois
Cihrcontribution2
Inst of Cancer Research~ $1,000~ $0~
