Grant: Project Grant

Age-related macular degeneration (AMD) is a leading cause of vision loss, yet its underlying mechanisms remain poorly understood. This project aims to leverage our preclinical research capabilities to enable advanced multi-mode optical coherence tomography (M2-OCT) imaging, allowing us to study the aging retina in unprecedented detail. We will utilize a multi-contrast functional retinal imaging system capable of switching between high-speed and polarization-sensitive modes. This will help us examine the structure and function of retinal layers, including photoreceptors, retinal pigment epithelium (RPE), and choroid, which are critical in AMD progression. Integrating the advanced retinal imaging techniques, we will construct a novel approach to precisely diagnose AMD based on functional retinal imaging. Specifically, using the M2-OCT system, we will investigate: 1. Aging effects on Photoreceptor-RPE-Choroid (PRC) complex • Photoreceptor function - measuring how retinal cells respond to light at different ages using a new optoretinography protocol. • RPE atrophy - assessing changes in polarization properties to track age-related RPE degeneration. • Choroidal blood flow - monitoring blood flow changes in the choroid, the layer supplying oxygen to the retina, to understand its role in AMD. 2. AMD-specific PRC complex changes using AMD mouse models - genetically modified mice and a laser-induced injury model to mimic the disease progression. By combining multi-mode retinal imaging with advanced image analysis, this research will provide new insights into how aging affects the retina and help identify potential targets for early and precise diagnosis as well as treatment of AMD.